| Atherosclerosis is a chronic inflammatory disease, ox-LDL plays a critical role in AS. LOX-1 as the new discoverable receptor of ox-LDL, participates in the initiation and development of AS through mediating the process that ox-LDL stimulate cell express kinds of inflammatory factors . Fractalkine is a unique chemokine in that it not only acts as a chemokine,but also as an adhesion molecule,FKN maybe play a important role in the process of VSMC migrate to atherosclerotic plaque,but the effect of FKN in AS hasn't be known.Apocynum venetum has lots of effects in the cardiovascular system,but researchs on the role of its inhibiting AS on the animal model level are more than on the cells level. In this study ,we will detect the expressions of FKN in the human aorta atherosclerotic lesion;to construct AS model through ox-LDL induce VSMC and detect which ox-LDL affect the expressions of FKN and the role of LOX-1 and Apocynum venetum,to discuss the role of FKN and AV in the inflammation of AS .That can offer the evidence in therapy.objective:1.To research the expressions of FKN in the human aorta atherosclerotic lesion. 2.To research the expressions of FKN by ox-LDL induced in AS and the mediated role of LOX-1. 3.To research the role of Apocynum venetum in AS and inflammation on the cell level .Method:We detected the expressions of FKN in the human aorta atherosclerotic lesion with immunohistochemistry;Smooth musclecell were incubated and treated with 100mg/L ox-LDL and different concenteations (0.2, 0.4, 0.8mg/mL) of AV and inhibitor of LOX-1 carrageenan(250ug/ml)for 24 hours.Then we detected the expression of LOX-1,FKN with ELISA and RT-PCR.Result:1. Compared with control group, the more expressions of Fractalkine have statistical significance (P<0.05) in the human aorta atherosclerotic lesion.2. After Incubating SMC with 100mg/L ox-LDL and different concenteations (0.2, 0.4, 0.8mg/L) of AV and carrageenan (250ug/ml)for 24 hours, compared with control group,ox-LDL can induce the expressions of LOX-1 , carrageenan and AV can significantly decrease the expressions of LOX-1 (P<0.05). The effect of AV1 group is more infirmly than AV2 and AV3 group,this effect is in a concentration dependent manner.3. After Incubating SMC with 100mg/L ox-LDL and different concenteations (0.2, 0.4, 0.8mg/L) of AV and carrageenan (250ug/ml)for 24 hours, the result of RT-PCR show: compared with control group, ox-LDL can induce the expressions of FKN( P<0.05 ) .Compared with ox-LDL group,carrageenan can significantly decrease the expressions of FKN (P<0.05), only 0.8mg/ml concenteations of AV can significantly decrease the expressions of LOX-1 (P<0.05).Conclusion:1.Fractalkine is the high expressions in the human aorta atherosclerotic lesion.2. ox-LDL can induce the high expressions of LOX-1 inVSMC,AV can inhibit this process in a concentration dependent manner.3. ox-LDL can induce the high expressions of FKN in VSMC.4. Inhibitor of LOX-1 carrageenan can decrease the expressions of FKN, it means that LOX-1 can mediat the process of which ox-LDL can induce the high expressions of FKN in VSMC.5. 0.8mg/ml concenteations of AV can decrease the expressions of LOX-1,FKN simultaneously,it means that the high concenteations of AV can inhibit the process of which ox-LDL can induce the high expressions of FKN in VSMC.In all, ox-LDL has the important role in AS, the receptor LOX-1 of ox-LDL can mediat the process of which ox-LDL can induce the high expressions of FKN in VSMC,thereby it participates in the certain process of AS.AV can inhibit the inflammation process of AS through inhibiting the expressions of FKN in VSMC by ox-LDL induced . That maybe offer the new direction in treating AS.
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