Study On The Expression Of NF-KappaBp65 And UPA In Gastric Carcinoma And Correlation With Clinicopathological Features

Objective: To investigate the expression of NF-KappaBp65 and uPA in human gastric carcinoma and normal gastric tissue and evaluate their functions, relation in gastric carcinogenesis , relation with clinical pathological features.Methods: Harvested 56 surgical gross samples and related clinical materials from patients with gastric carcinoma,who were not treated by radiation,chemotherapy and any other special treatment.Totally there were 56 gastric carcinoma tissue cases and 10 normal gastric tissue studied .According to the 《NEW DIAGNOSTIC and THERAPEUTICAL STANDARD of COMMON MALIGNANCE》 , the 56 cases consisted of 19 well and moderately differentiated cases,37 poorly differentiated and mucinous cases in terms of histological classification. On the basis of the biological characterization ,in the 56 cases ,43 were with lymph node metastasis, while 13 cases were without metastasis.Depth of invasion to mucosa/submucosa, muscularis and serosa were 2, 17 and 37 respectively. In the 56 , 22 were in sinus ventriculi, 6 in corpus ventriculi and 28 in fundus and cardia ventriculi.Tumoer size that was smaller than 5cm were 34 cases,while that was bigger than 5cm were 22 cases.At the same time, harvested 10 samples of normal gastric tissue as control . Immunohistochemistry was imployed to detect the expression of NF-κBp65 and uPA in all of samples.Results: 1. The expression features of NF-κBp65 in gastric carcinoma.(1) 10 normal gastric tissue cases were almost negative for the expression of NF-κBp65 .But a relatively high positive expression rate was found in gastric carcinoma.Of the 56 patients with gastric carcinoma,50(89.3%) were expressed positively for NF-κBp65 , which had obvious statistical significance (P<0.01).