| In this project, the chemical constituents of Serissa serissoides and some biological activities, such as the antiviral activities and anti-cancer activities, were studied.Ten compounds and one sterol mixture were isolated from Serissa serissoides by various chromatographic methods such as silicon gel column chromatography, Sephadex LH-20, high speed countercurrent chromatography (HSCCC), assistant with traditional methods like extraction and recrystalization. The structures of the nine compounds had been identified on the basis of spectral and chemical evidence. They are palmitic acid (1), corosolic acid (2), daucosterol (3), urs-12-en-28-ol (4), oleanolic acid (6), 8-sitosterol (7), uroslic acid (8), 4-hydroxy-3- methoxy- benzoic acid (9), 2,6-dimethoxy-p-benzoquinone (10), and an aliphatic hydrocarbon (5). Based on GC-MS analysis, the sterol mixture had been identified as campesterol, 4, 4-dimethyl-cholesta-22, 24-dien -5-ol and β-sitosterol, among which 4,4-dimethyl-cholesta -22,24 -dien-5-ol and B-sitosterol were the major components, and their contents in the mixture were 37.84% and 45.17%, respectively. The compounds were first isolated from the Serissa Comm expect daucosterol, uroslic acid and oleanolic acid.The inhibitory activities of the ethanol extract, fractions and some isolated compounds against Cox B3 and HSV-1 in vitro were studied by cell culture methods. The cell toxicities of the samples were tested by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphe-nyltetrazolium bromide (MTT) assaying. The inhibitory effects were evaluated by cytopathic effect (CPE) reduction assaying. The results indicated that all the samples did not show any obvious antiviral effects in the range of the experimental concentrations.The inhibitory activities against tumor cells (Hep-2, H460, A549, BEL-7402, Lovo) in vitro were also studied by cell culture methods. The cell toxicities of the...
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