| Objective To investigate the effect of retinoic acid (RA) nanoparticle eyedrops as therapeutic agent whether it is beneficial in prevention of posterior capsular opacification (PCO) and the toxicity of the drug.Methods 1.Eighteen New Zealand albino rabbits were divided randomly into three groups, and were performed the extracapsular cataract extraction(ECCE). Group A: control group. Group B: using RA nanoparticle eyedrops(300μg/ml). Group C: using RA nanoparticle eyedrops(700μg/ml).The changes of the posterior capsule were observed by slit-lamp microscope, ophthalmoscope , photography,and electron microscopy.2.All the right eye of the ten cat were treated with the balanced salt solution(BSS),and all the left eye of the ten cat were treated with RA nanoparticle eyedrops(700μg/ml) t.i.d for 12 weeks.Then the endothelium of the cornea were stained with trypan blue and alizarin red immediately after the corneal excision.The morphology of the cornea,iris and ciliary body was observed with microscope. Results 1.At 12 weeks after operation, the occurrence of posterior capsular opacification was noticed in 10,6,4 eyes respectively in the 3 groups. The PCO score and PCO rate of the control group were significantly higher than those of the RA groups(P<0.05). Histological analysis showed that the proliferative activity of the control group was much more intense than that of the RA groups. 2.The corneal endothelial cells of the two groups presented regular intercellular borders.The structure of the corneal was intact.The iris and ciliary body got no chronic inflammation.Conclusion The RA nanoparticle eyedrops can inhibit metaplasia and proliferation of lens epithelial cells in rabbit eyes without obvious toxicity. It could be used as a potential agent in the prevention of the development of PCO.
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