The Effect Of PGMS On Growth Of Rabbit Lens Epithelial Cells Based On The Rabbit Capsular Bag In Vitro

Objective: To investigate the inhibition of rabbit lens epithelial cell survival and growth by PGMS on the rabbit capsular bag in vitro.Methods: Capsular bags were prepared from rabbit eyes after extracapsular cataract extraction(ECCE) and incubated in various concentration of PGMS(0.2mg/ml, 0.4 mg/ml, 0.8 mg/ml) during various incubation periods(2min, 5min, 10min). After treatment, the capsular bags were cultured for 7 days in Eagle's minimal essential medium supplemented with 5% fetal calf serum. The specimens were fixed in neutral buffered formalin/glutaraldehyde and examinated with light microscopy and transmission electron microscopy(TEM). Capsular bags without receiving PGMS only served as controls.Result: PGMS inhibited the proliferation of RLECs with themanner of concentration and time dependentment. At the threshold protocol of incubation in PGMS at 0.8mg/ml for 5min, proliferative activity of cells were largely arrested and nearly no RLECs was seen on the posterior capsule. Control group had no effect on structure and proliferative activity of RLECs, and the growth proceeded rapidly so that the posterior capsule were totally covered by a confluent monolayer of cell by the end of 7 day. Under TEM, the cells in the control group were tightly arrayed with clearly defined celluar boundary and structure; while celluar deformity and undefined intracelluar structure could be seen in the 0.4 mg/ml and 0.8 mg/ml experimental groups.Conclusion: PGMS can effectively suppress the proliferation of RLECs and be a promising means of preventment of posterior capsule opacification.