| CRF, chronic renal failure, means nephron lesion and renal function damage, resulted from primary or secondary renal diseases. It induces a series of metabolic disorder and a group of clinic symptoms. Hemodialysis and kidney transplantation play a very important role in the terminal treatment of CRF, while not in the early and middle stage prevention and cure. The renal diseases are relatively frequent; no matter what kind of pathogeny, whether are induced by immunologic mechanisms or not, if can not be treated timely and effectively, when the lesions of renal parenchyma accumulate to certain extent, they are all progressive and irreversible as CRF. Thus, in the world there are many patients of early CRF, several time of hemodialysis ones, urgently need therapies to delay or stop the progression of CRF.It has been proved that Cordyceps sinensis(Berk.) Sacc and its preparations not only help to delay the progression of CRF, but also alleviate the side effect of hemodialysis, accordingly improve the existent qualification of CRF patients. But Cordyceps sinensis is too precious to apply widely, because of its limited natural sources and difficult to ferment. Cordyceps militaris (L.ex Fr) Link is similar to C. sinensis on life history, chemical components, pharmacological actions, and so on. But it is only developed as a kind of common hygienical food. Moreover there is no report on whether C. militaris can anti the CRF yet.Objective: to confirm what is the effective component of C. militaris has anti CRF function .Methods: Hot water extracted polysaccharide from fruit body of C. militaris; coarsely isolated it by deposited it in different concentrations of ethanol from the aqueous extracts. Protein removed using Sevag method. Phenol- Sulfuric acid method determined the total carbohydrate content of sugar; carbazole method measured glucuronic acid content; Bradford method examined the quantity of been removed protein. Applied Sephacryl G-75 gel column to further isolate and purify polysaccharide, and determined their molecular weight. DNS method measured reducing sugar content of coarse polysaccharide components; Pyrogallol autooxidation method and Fenton reaction to detect the antioxidant activity of them, to speculate about their anti CRF mechanism.mensurated the Median Lethal Dose and Maximum Tolerance Dose of raw material fruit body of C. militaris on Kunming mice by i.g. Treated Wistar rats 1weeks respectively with uremic clearance granule, Three deferent doses of raw polysaccharide of C. militaris, aqueous extract from the fruit body of C. militaris, and polysaccharide deposited by ethanol from the aqueous extract. Get the blood of the rate ,at 2 hours after the last i.g .Then culture the MC by the medium cotains the blood , Observice the effect on Cell Proliferation.Conclusions:We got 5 polysaccharide:CP-1 from 80%deposited by thanol from the aqueous extract. CP-2, CP-3,CP-4,CP-5 from 50%deposited by ethanol from the aqueous extract . From the pharmacology experiment wo found the CP-1 is what we want.The research results indicate that the CP-1 polysaccharide is the one certain anti CRF activity, which maybe relative to its antioxidation. CP-1 polysaccharide's mainly composed of neutral polysaccharide components, while lack of acid ones. The molecular weight is about 17KDa. And it shows quite high antioxidant activity.The research supplies academic supports for C. militaris further development. aqueous extract from the fruit body of C. militaris is no side-effect, and safety. Therefore, it is more beneficial for the CRF patients'long-term administration. It`s Suppression on Cell multiplicationcan improve CRF prognosis, which provides it a wide prospect in clinical application.
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