Expression And Significance Of Maspin In Cervical Squamous Carcinoma And Cervical Intraneoplasm

Objections To investigate the expression of Maspin and the possible relationship between Maspin expression and clinicopathological parameters in cervical squamous carcinoma , and evaluate its possible role in tumorigenesis and progression of cervical carcinoma.Methods Maspin expression was detected in normal cervical tissue (n=12), cervical intraneoplasm (CIN )(n=46) and cervical squamous carcinoma (n=63) by immunohisto-chemistry. And analyze the relation to age , clinical stage , muscular invasion , histological grade and lymph node metastasis.Results (1) The abnormal rates of Maspin expression were 25.00% (3/12 ), 36.96% (17/46 ) and 71.43% ( 45/63 ) in normal cervix tissue, CIN and cervical squamous carcinoma, respectively. And were 30.77% ( 4/13 ), 36.84% ( 7/19 ), and 42.86% ( 6/14 ) in CIN I , II and III, respectively. (2) Cervical squamous carcinoma less frequently expressed Maspin than the normal cervical tissue and CIN ( P<0.05 ). (3) In 63 cases of cervical squamous carcinoma , the abnormal expression rate were 70.27% ( 26/37 ) in < 48 years of age , 73.08% ( 19/26 ) in > 48 years of age , there was no significant difference ( P<0.05 ) ; 52.63% ( 10/19 ) in stage I , 62.50% ( 5/8 ) in stage II , 83.33% ( 30/36 ) in stage HI ; 41.18% ( 7/17 ) in G₁, 81.08% (30/37) in G₂, 88.89% ( 8/9 ) in G₃ ; 42.86% ( 6/14 ) in non muscular invasion , 61.54% ( 8/13 ) in microinvasion, 74.0% ( 37/50 ) in higher muscular invasion ; 43.75% ( 7/16 ) in negative lymph node metastasis , 72.73% ( 8/11 ) in positive lymph node metastasis ; significant difference were exist in each group ( P<0.05 ). (4) Maspin expression showed negative association with the clinical grade, cell differentiation and lymph nodes metastasis ( P<0.05 ) , however there were no correlated with different age ( P > 0.05).Conclusions Down-regulated expression of Maspin plays an important role in cervical carcinogenesis. Abnormal expression of maspin might have inhibitory effect on invasion and metastasis of cervical carcinoma and acts as an effective and objective marker to indicate the pathobiological behavior of cervical carcinoma.