The Experimental Study On The Effects Of Acupuncture Sishencong To Electroenphalogram Of Normal Rats And NE,DA In Insomnia Big Mouse Plasma

Purpose: (1).To clarify the effects of electro-acupuncture(EA) SiShenCong on mice' sleep time and sleep phrase.(2).To study the mechanisms of EA on insomnia mice and probe into the effects EA SiShenCong on NE. DA of insomnia mice in peripheral blood plasma, to offer the objective basis for the treatment of insomnia with acupuncture SiShengCong.Methods: (1). Using 16 physiological signal recording instrument record the big mouse sleep time, record the Electroencephalogram .Observe the time and phase changing of mice.(2)This text regard Wistar mouse as targets, choosing 50 big mice which are divided into 5 groups at random.Blank group (10); Model control group (10) ; Diazepam group (10) ; Hand acupuncture group (10); Electro-acupuncture group (10). Adopt abdominal cavity inject and prepare insomnia animal model to PCPA through 7 day acupuncture and medicine group to irritate diazepam into the stomach of mice in 7 days, fetch the blood of big mice' eyeball, determine NE and DA content in the blood plasma through the fluorescent law after PCPA abdominal cavity injection.Results: One(1.) There was significant difference in mice sleep time among EA group and hand acupuncture group(P<0.05) comparing with model control group. (2)Compared hand acupuncture group with electric acupuncture group, the sleep time of big mice was lengthened more obviously. There have a significant difference in two groups (P<0.05). (3) Compared hand acupuncture group and electric acupuncture with model control group, the slow wave sleep 2(SWS2) ,REM and TST was obviously extended .There is a significant difference (P<0.05). Among the three groups, there is no significant difference (P> 0.05). (4) Compared electric acupuncture group with hand acupuncture group, the sleep phase of SWS2, REM and TST was lengthened obviously. There is a significant difference (P<0.05). (5) Compared hand acupuncture group and electric acupuncture group with model control group, TST was lengthened.The electric acupuncture group was lengthened more obviously, there is a significant difference (P<0.05).Two. (1)Compared model control group with blank group, NE and DA level is obviously higher than the blank group in the blood plasma (p<0.05);(2)In hand acupuncture and electro-acupuncture group, the NE and DA competence in blood plasma was obviously reduced. Compared with model control group , there is prominent difference (p<0.05); (3) Compared electro- acupuncture group with hand acupuncture group , NE and DA content in blood plasma was reduced more obviously, there is prominent difference (p<0.05); (4)There is not obvious difference between model control group and diazepam group (p> 0.05).Conclusions :( 1) Acupuncture can. lengthen the sleep time of mice, can promote the sleep. (2)Acupuncture can adjust the big sleep phase of mice, SWS2 ,REM and TST was extened,the proportion of sleep was increased.The electric acupuncture group has great effects (3) Acupuncture can reduce NE,DAcontent of insomnia mice.(4) Diazepam influenced on NE, DA content of blood plasma to PCPA insomnia mouse.(5) Electric acupuncture has great improvement to insomnia mice . (6)In the two experiments, there is great effects of acupuncture SiShenCong on the insomnia mice. The mechanisms of the case is that with changing mouse sleep structure , there probably has relationship with the neurotransmitter .