| Objective:Through the collection of clinical cases and animal experiment, the analysis for purging the curative effect of acupuncture treatment of ischemic stroke patients with insomnia after 5 - HT in serum, animal model and 5-HIAA neurotransmitters, explore its molecular mechanism.Methods:Clinical research and animal experimental study this topic research is divided into twoaspects:I. clinical researchIn total 70 cases, random method, divided into (1) acupuncture group, (2) in the control group, each group of 35 cases. Subjects dropped out and eliminate a total of 10 cases, cases finally collected 60 cases, including (1) acupuncture group and control group, 30 cases (control group 30 cases.Interventions:1. basis for interventionAll the patients were in stroke medicine routine treatment. Symptomatic treatment and support therapy, including the control of blood pressure, blood glucose, blood lipid, body temperature, oxygen, maintaining water, electrolyte and acid-base balance, to aspirin and use can improve the blood circulation in the brain and the brain cell activator, etc. All patients do basic rehabilitation therapy (including good limb position put, bedside table, horizontal movement of balance, balance training, bed stand, main passive movement, homework therapy, etc.).2. acupuncture groupDeficiency syndrome:use reinforcing acupuncture technique;find out:will, four gods, vasomotor area, god door, zusanli, taixi; Operation:the supplement wildfires burning method is adopted in zusanli, other points with mild reinforcing and attenuating, when the patient appear acid bilges retaining needle after 30 minutes;Empirical syndrome:use reducing acupuncture technique; find out:will, four gods, vasomotor area, taichong, qiuxu, daling; Operation methods:cold day type reducing method in qiuxu point, other points with mild reinforcing and attenuating, when the patient appear acid bilges retaining needle after 30 minutes;Needle selection of diameter of 0.25mm, long more than 40 mm han brand stainless steel needle method 1 times a day; Treatment time in 3 to 6 PM, treatment for 10 days in a row,5 days a week, lasting 2 Week. At the end of the observation curative effect.3. the control groupOral estazolam.1 mg each time, every night before sleeping,1 times a suit of total 10 days,5 day a week, lasting 2 week, at the end of the observation curative effect.Before, stop on the first day after the treatment, the fifth day record patients’clinical symptoms, fill the Pittsburgh sleep quality experienced sleep quality and sleep monitoring.II. animal experimental studySPF 84 healthy SD rats were divided into four groups (1) normal group, (2) the model group, (3) acupuncture group and western medicine group.Building methods:using intravascular line blocking method of manufacturing a stroke model, tied to a stroke model by intraperitoneal injection of again PCPA manufacturing insomnia after ischemic stroke.Interventions:(1) normal group, (2) model of insomnia group empty grasp, will not be any processing. (3) acupuncture group:will, four god astute. Day at a time,5 times as a course of treatment. A total of 2 course of treatment. (4) the western medicine group:estazolam treatment for 3 days. According to adult hypnotic dose omg/day,1 rat of choose and employ persons to convert formula to calculate the rat medicine dose body surface area to the dose of 0.92 mg/kg. The daily morning 9:00-10:00 to rats lavage.(2) Results:1. Both reinforcing or reducing acupuncture technique and oral estazolam can reduce ischemic cerebral apoplexy patients with insomnia after TCM syndrome integral.2. Both reinforcing or reducing acupuncture and oral estazolam can reduce ischemic cerebral apoplexy patients with insomnia after the Pittsburgh sleep quality index.3. After ischemic stroke in patients with insomnia symptoms scale (TESS) score, compare to oral estazolam treatment,the acupuncture manthod in above has lower score, it means fewer side effects.4. Consult reference state drug administration issued the "Chinese medicine new medicine clinical research guiding principle" curative effect evaluation standard, estazolam treatment for ischemic stroke after insomnia curative effect is better than acupuncture.5. Intravascular tied line blocking method and PCPA abdominal cavity injection method can manufacture can insomnia animal model of rats after ischemic stroke.6. Acupuncture and intraperitoneal injection of estazolam can improve animal model 5-HT and 5 - HIAA content in serum, the effect of them has been confirmed.Conclusion:1. Reinforcing or reducing acupuncture can alleviate state of patients with insomnia.It’s the first step of researching the fundamental Chinese medical theory about proper acupuncture treatment based on syndrome differentiation could be better than in randomly or not.2. Reinforcing or reducing acupuncture treatment reduce the side effects of insomnia after stroke, it has better curative effect than estazolam.3. by intraperitoneal injection of intravascular tied line blocking method and PCPA hair may be effective insomnia after stroke model building method.4. Acupuncture can improve the animal model of 5 - HT and 5 - HIAA content in serum.lt is the animal experiment evidence of acupuncture can change the central neurotransmitter, also it is the fundamental animal experiment to prove the theory about proper acupuncture treatment based on syndrome differentiation could be better than in randomly or not. |
PDF Full Text Request