Effects Of Electro-Acupuncture On Nerve Growth Factor In Rats With Experimentally Induced Polycystic Ovaries

Objective:1,The first part of the study investigated dose-response the discovery of the exact dose of EV needed to produce fully developed polycystic ovaries.2,The second part of this study investigated treatment with EA-what contribution NGF made to the etiology and maintenance of EV-induced PCO in rats and if and to what extent EA has an effect on NGF and ovarian morphology in experimentally introduced PCO.Methods: 1,Thirty rats were injected with one of two different doses of EV in an oil solution or with oil alone to ascertain the optimal dose for induction of PCO.2,The optimal dose (4 mg EV in 0.2 ml oil/rat) and timing(30 days after injection) were chosen for the experiments. In total, 30rats took part. Ten rats in the EV control grojup and ten in the EA-treated EV group were injected i.m. with 4 mg EV in 0.2 ml oil/rat, eight rats in the oil control group with 0.2 ml oil, and ten rats in the Normal group with nothing. All 30 were decapitated on Day 30 after injection, that is, two days after the last EA treatment. The EA-treated EV group was subjected to 14 EA treatments every second day, beginning 2 days after the i.m. injection of EV. The needles were inserted with a low burst frequency of 2 Hz. The location and type of stimulation were the same in all rats.Conclusions:1,4 mg EV in 0.2 ml oil/rat and 30 days after injection produce fully developed polycystic ovaries.2,The conclusion of this study is that repeated EA treatments reduce ovarian NGF concentrations to within normal ranges.