Expression And Significance Of PIK3CA, PTEN In Uterine Cervical Neoplasm

Objective The purpose of this study was to explore PIK3CA oncogene and PTENtumor suppressor gene expression in uterine cervical carcinoma and cervicalintraepithelial neoplasia(CIN) lesions.Methods RT-PCR was performed to determine the expression of messenger RNA(mRNA) of PIK3CA and PTEN in 30 uterine cervical carcinoma and 16 normalcases. The normal cases and 20 of these 30 uterine cervical carcinoma wereselected; In addition, 25 CINâ…¡-â…¢,10 CIN and 15 normal cases were selected.Immunohistochemical staining with coresponding antibodies were performed toanalysis the protein expression of PIK3CA and PTEN.Result 1. There were 12 cases of amplification of PIK3CA mRNA and 10 cases ofloss of PTEN in all 30 uterine cervical carcinoma. But there is none case of normalcase expressing amplification of PIK3CA or loss of PTEN. The expression ofPIK3CA and PTEN mRNA were significantly different between tissue of normalcervix and the cervical neoplasm(t=2.672, P＜0.05; t=-3.336, P＜0.05).2. The expression of PI3K p110αwere significantly different in normalcervix, CINâ… , CINâ…¡-â…¢and uterine cervical carcinoma(F=7.159, P＜0.01)。Aftermultiple comparisons we can conclude though there was no difference betweennormal cervix and CINâ… (P＞0.05), CINâ… , CINâ…¡-â…¢and uterine cervical carcinomawere difference between each other (P＜0.05).3. The positive rate of PTEN were 100ï¼…,92.0ï¼…,75.0ï¼…respectively innormal cervix and CINâ… ,CINâ…¡-â…¢,uterine cervical carcinoma. We can concludethat it reduced in the progression of cervical neoplasm. The difference between eachother is significantly (F=6.151, P＜0.01).4. PTEN, but not PI3K p110a, was highly associated with tumordifferentiation grade of uterine cervical carcinoma (F=5.998, P＜0.05).The expressionof PTEN in low degree of differentiation group was significantly higher than that inhigh and middle degree of differentiation.5. There was a positive correlation between PIK3CA and PTEN expression(r₁=0.345, p=0.019; r₂=0.419, p=0.000). Tumors with overexpression of PIK3CAhad normal PTEN expression. Yet tumors with deletion of PTEN had normal PIK3CAexpression.conclusion Our results suggests that the gene aberrant of PIK3CA and PTEN werecorrelated with the cervical cancer. Tumor progression in the cervical epithelium isaccompanied by increase of PIK3CA or loss of PTEN protein expression, which ismutually exclusive events in cervical tumorgenesis.