The Autoimmune Response Of Nucleus Pulposus And Its Significance Of Forensic Medicine

IntroductionIn the clinical forensic medicine appraisal work, involves the Lumbar intervertebral disc herniation (LDH) case to be many. At present appraise in the work about the LDH main basis clinical manifestation, the imageology as well as the neuroelectricity physiology technology. However as a result of the clinical manifestation subjectiveity, the imageology only can merely reflect morphological change, the neuroelectricity physiology technology stresses on the nerve root function condition appraisal, has certain limitation respectively. In recent years, the LDH immunology aspect hopefully provided new method for the Lumbar intervertebral disc retrogression change and the LDH distinction and the LDH morbidity time forensic medicine appraisal.The nucleus pulposus mature then the blood vessel degenerates since the embryonic development supplies bloodless, is wrapped with the blood and the lymphatic system by the annular f韇rosus isolates, Only completes with the outside material exchange through the cartilage lamina erminalis osmosis. The unique dissection structure and the material interchange way caused it to have own antigen foundation. In pathology situations, the nucleus pulposus organization contacts the immune system, causes autoimmune reactivity.This research uses the New Zealand Big Ear Rabbit nucleus pulposus organization immunize homogeneous rabbit to prepare the antiserum, immunity enzyme dot technology (Dot-ELISA) detect immunity and the experimental group and control group blood serum IgG and IgM immune body titer change situation. Thus examines nucleus pulposus whether has own immunogenic, discusses the anti-nucleus pulposus immune body titer change to be at the forensic medicine appraisal Lumbar intervertebral disc herniation.Materials and Methods1．The New Zealand big ear rabbit, the male gender, the state of health is good, body weight 2．0-2．5 kilograms, age of the moon in six month.2．Under the aseptic condition extract the New Zealand Big Ear Rabbit Nucleus pulposus Organization refine, centrifugate the suspension does for the antigen solution.3．Experimental group hypodermic injection nucleus pulposus organization suspension and the adjuvant emulsifier immunize new Zealand big ear, the physiological saline control group inject physiological saline and the dosage same with experimental group, the adjuvant control group injects adjuvant and the dosage same with experimental group, the experimental technique are same.4．Immunity enzyme dot technology examination blood serum IgG and IgM immune body titer change and degree.5．Chi-square test analysis blood serum IgG and IgM immune body titer change and experimental group and control group blood serum IgG and IgM immune body titer change difference whether have statistic significance.Results1．Square matrix experiment screens the IgG immune body most suitable experimental condition, the coat of antigen concentration is 50(?)g/ml, after the immunity in the blood serum 1:1-1:128 dilution scopes, the nitrocellulose membrane colored effect is good, along with the blood serum in the immune body concentration drop, the color presents the progressive degression tendency.2．Square matrix experiment screens the IgM immune body most suitable experimental condition, the coat of antigen concentration is 40(?)g/ml, after the immunity in the blood serum 1:1-1:32 dilution scopes, the cellulose nitrate membrane colored effect is good, along with the blood serum in the immune body concentration drops, the color presents the progressive degression tendency. 3．Take the nucleus pulposus organization suspension as the antigen immunize rabbit, after the immunity, in the blood serum IgG and IgM titer compares before the immunity to elevate, but in the blood serum the IgG immune antibody titer is 1:128, the IgM immune antibody titer is 1:32, the two ascension degree has the big difference.4．The nucleus pulposus organization suspension compares after antigen immunity experimental group and the physiological saline control group and the adjuvant control group, in the experimental group blood serum IgG and IgM immune antibody titer compares the control group to elevate.DiscussionWhen the antigen the corresponding immune body member proportion is most appropriate, they only then meet each other to connect mutually have the complete main body structure immunity compound giant grid shape aggregate, causes the protein member the sparse water structure full exposition, the immune body and antigen combine most reliable. After the square matrix tests to know that, the coat antigen concentration is 50(?)g/ml, the blood serum dilution is 1: 128 for examine the IgG immune body the best experimental condition. The coat antigen concentration is 40(?)g/ml, the blood serum dilution is 1:32 for examines the IgM immune body the best experimental condition.The finding according to (?)=0．05 examinate, the immunity in the blood serum IgG and IgM immune body titer change and the experimental group and the control group blood serum IgG and IgM immune body titer change difference have statistics significance. Those show nucleus organization has own immunogenic, once contact organism immune system, may stimulate organism to have the immunity reply, causes in the blood serum the anti-nucleus pulposus IgG immune body, the anti-nucleus pulposus IgM immune body titer ascension.The nucleus organization antigen ingredient is complex, has the high nonuniformity, part are not the nucleus organization unique, has same or the similar antigen with the rabbit in vivo other organization organ; Or in the nucleus pulposus organization includes the specificity protein antigen ingredient has many antigen decision bunch, organizes the organ with in vivo other the antigen decision bunch same or similar, in the blood serum exists the immune body the antigen ingredient have the overlapping response with the nucleus pulposus. Then has not passed through in the immunity rabbit blood serum the immune body and the nucleus pulposus organization can have the response, appears masculine or the weak masculine change.To remove infection and other own immunity disease in the immune body titer ascension situation, According to the blood serum in the immune body titer change situation, may carries on the distinction prominently to the lumbar intervertebral disc retrogression change and the LDH. Dynamic examination LDH patient blood serum IgG and IgM immune body titer change, hopefully to the early stage breakage lumbar intervertebral disc herniation morbidity time makes the preliminary inference.ConclusionThe nucleus pulposus has own immunogenic, after organism immune system contact, may stimulate organism to have the immunity reply, causes in the blood serum IgG and IgM immune body titer ascension.Based on the immunology immune body expression rules, lays the immunology rationale for the forensic medicine distinction lumbar intervertebral disc retrogression change and LDH and the LDH morbidity time.