The Effect On Basic Fibroblast Growth Factor And Microcirculation Of Glucocorticoid-induced Avascular Necrosis Of Femoral Head In Rabbits By Treatment Of Tao Hong Si Wu Decoction

Purpose: To investigate the effect on basic fibroblast growth factor,microvessel density and hemorheology of glucocorticoid-induced avascularnecrosis of femoral head in rabbits by treatment of tap hong si wu decoction. Tointerpret the mechanism of the effect on glucocorticoid-induced avascularnecrosis of femoral head by activating blood circulation, and offer a effectivemethod to clinical.Method: 60 rabbits were randomly divided into 2 groups: 6 rabbits were in commongroup and 54 rabbits were in experimental group, the rabbits in common groupwere as normal control; the rabbits in experimental group were administered withhydroxyprednisone through gluteus injection (twice a week) for 6 weeks as control: there were 6 rabbits were killed in each group after 6 weeks, to be assure thatthe model were succed. All surplus rabbits were divided into healing group andcontrol group: the healing group were administrated with tap hong si wu decoction7ml/kg per day, the control group were administrated with sodium chloride 7ml/kgper day. then, after 1,2 and 4 weeks, killed the animal, and detected all indexes.Results:①the result electron microscope showed: in the experiment group, bonecell decreased, cataplasised and pyknosised, lipid droplet emerged, the numberof osteoblast decreased, adipocytes in the camera pulpi accreted, bone cells inthe common group contained large number of rough endoplasmic reticulum andpolysome, bioblast becameprosperous, the architecture of the crista was lampros, kernel heterochromatin were plentiful, nucleolus could be seen.②histomorphologic measurements showed that empty lacuna increased and trabeculaof bone decreased more significantly in control groups and than those in healing group and the medullary cavity lipocytes in control group showed larger sizeand more amount than those in healing group.③whole blood viscosity, plasmaviscosity and fibrin in control groups increased significantly compared withthose in healing groups.④the expression of bFGF in serum measured byradioimmunity reinforced significantly in healing groups compared with controlgroups.⑤microvessel density(MVD) detected by immunohistochemistry: the MVD offemoral head in healing groups increased day by day, the MVD of femoral headin control groups degraded significantly compared with healing groups.Conclusion: The administration of large dose glucocorticoid can induced avacularnecrosis of femoral head. the bone tissue and the bone cell of the femoral headand the hemorheology were changed abnormality, the condition could be improvedand the expression of bFGF could be reinforced by tap hong si wu decoction, atthe same time, it facilited the regeneration of capillary vessel and improvedthe condition of microcirculation, maybe that was one reason why tap hong si wudecoction effect on glucocorticoid-induced avacular necrosis of femoral head.