The Effect Of Mycophenolate Mofetil On The Expression Of RANTES And ED-1 In The Diabetic Rats

Objective: To observe the expression of RANTES,ED-1,CoⅠandCoⅣin the renal tissue of diabetic rats intervening by MMF, and exploreif MMF can inhibit inflammatory reaction to protect kidney.Methods: Seventy-two male Sprague-Dawley rats were randomlydivided into three groups after uninephrectomy: normal controlgroup(n=24), diabetic model group(n=24), MMF-treated group (n=24).Four weeks later, model of diabetic mellitus were induced bystreptozotocin(60mg/kg). MMF-treated group was treated with MMF(15mg/kg/d) after the models were successfully established. At 4,6,10,14 weeks, six rats of each group were killed to test 24hr urinary proteincount. The left kidneys were taken out. The histological changes of theleft kidneys were observed after HE and Masson stain. The expression ofRANTES, ED-1, CoⅠand CoⅣprotein in renal tissue were detected byimmunohistochemistry. The expression of RANTES mRNA in renaltissue was detected by RT-PCR.Results: 1.Compared with the normal control group, 24hr urinaryprotein count increased significantly in the diabetic model group(p＜0.01) .2. Compared with the normal control group, diabetic ratsdisplayed glomerulus hypertrophy, renal tubule cellula epithelialisdegeneration or defluxion, histoleucocyte/macrophagus infiltration,extracelluar matrix increasing, renal interstitial fibrosis, and more severitywith course of disease going on. 3.Compared with the normal controlgroup, the expression of RANTES, ED-1, CoⅠ, CoⅣprotein andRANTES mRNA in the kidneys of diabetic rats were increasedsignificantly (p＜0.05 or p＜0.01). 4.Compared with diabetic model group,24hr urinary protein count, the expression of RANTES, ED-1, CoⅠ,CoⅣprotein and RANTES mRNA in the kidney of MMF-treated grouprats were significantly decreased (p＜0.05 or p＜0.01).Conclusions: MMF may play a certain role in renoprotection in thediabetic model, and it may be through reducing the expression ofRANTES protein and RANTES mRNA and histoleucocyte/macrophagusinfiltrating in renal tissue, and inhibiting inflammatory reaction in the early stage.