The Association Of Regulated Upon Activation Normal T Cell Expressed And Secreted Gene Polymorphism With Type 2 Diabetic Nephropathy

Objectives: Inflammation reactions are associated with the occurrence and development of diabetic nephropathy. Regulated upon activation normal T cell expressed and secreted(RANTES), belonged to C-C subtribe chemokine , can be secreted by both mesangial cell of renal glomerulus and epithelial cells of renal tubule. RANTES is associated with the sclerosis of renal glomerulus and the f ibrosis of renal tubule interstitial. As showed recently,the single nucleotide polymorphism (SNP) in RANTES promoter -403G/A has an increase of RANTES gene expression. It is the aim of this study to investigate the distribution of the single nucleotide polymorphism in RANTES promoter —403G/A in the Han population in Tianjin, and to assess if it is possible that the distribution is associated with diabetic nephropathy in type 2 diabetes mellitus.Method: A total of 244 Han people was involved in the patients group(type 2 diabetes mellitus group) who are selected from the inpatients in Metabolism Disease Hospital, Tianjin Medical University. According to urine albumin excretion rate(UAER), it was classified into three groups, the normoalbuminuria group(DNO group, 86 people,34 male and 52 female, average age was 61.53 ± 8. 95), the microalbuminuria group(DNl group, 84 people, 44 male and 40 female, average age was 61. 85 ±10. 71), and the macroalbuminuria group(DN2 group, 74 people, 40 maleand 34 female, average age was 63. 35+10. 07). A total of 90 Han subjects was involved in the control group (38 male and 52 female, average age was 58.36 + 8.26). Clinical data was collected, and genomic DNA was extracted from peripheral blood cells using a Protease K Fasting Burst Technique. PCR-RFLP analysis was performed to screen the single nucleotide polymorphism in RANTES promoter — 403G/A. Finally, statistics anylysis was performed.Result: (1) The prevalence of the RANTES promoter - 403G/A genotypes and alleles in each group were consistent with Hardy-Weinberg equilibrium law. (2)A polymorphism of RANTES promoter —403G/A were screened both in the diabetes group and the control group. G/G> G/A and A/A genotypes and G^ A alleles were found. The frequency of the genotype and allele in the diabetes group was 50.4% for G/G, 35.7% for G/A, 13.9% for A/A genotype, and 31.8% for A allele. It was 41. 1%, 45. 6%, 13. 3% and 36.1% respectively in the control group. There was no significant difference between the two groups for the respective frequency of the genotypes and alleles(P>0. 05). (3) The frequency of the genotype and allele in the DNO was 59.3% for G/G, 26.7% for G/A, 14.0% for A/A genotype, and 27.3% for A allele. It was 53. 6%, 35. 7%, 10. 7% and 28.6% respectively in the DNl;And was 36. 5%, 45. 9%, 17. 6% and 40.5% respectively in the DN2. There was significant difference between the three groups for the respective frequency of the genotype and allele(P<0. 05). Followed by x2 division method, there was no significant difference between the DNO and DNl(P>0. 05). Merging the DNO and DN1 compared with the DN2 in result that significant difference were found between DN2 and DN0+DN1. A (+) genotype and A allele frequency in DN2 were higher than...