Expression Of Survivin, CerbB-2, Ki-67 In Endometrial Carcinoma And Its Clinical Significance By Tissue Microarray Technique

Objective: Endometrial carcinoma is one of the most frequent malignant tumors in female genital system. In recent years, there is an increasing incidence of endometrial carcinoma, 20%~30% in malignant tumors in female genital system, following with life lengthening and extensive application of estrogen. It is gradually realized that the development of tumors is associated with inhibited apoptosis, cell hyperplasy and oncogene activation and so on.Survivin, a novel anti-apoptotic gene found recently, can express a kind of proteins whose apoptosis suppression effect is the strongest up to now. It expresses in most carcinoma tissues and has the dual effects that can suppress apoptosis and promote cell proliferation. CerbB-2, a proto-oncogene, is in the quiescence condition at normal, which takes part in cell growth and cell differentiation regulation. After activated, it can promote the occurrence and development of tumor. Ki-67 is a kind of cell proliferation nuclear antigens. It exists in proliferative phase cells and is expressed in all cell cycles except G0 phase. Abnormally, it expresses in many tumors and precancerous lesion and is regarded as the marker of cell proliferation activity.The objective of this study is to detect the expression of Survivin, CerbB-2 and Ki-67 in endometrial carcinoma by tissue microarray and immunohistochemistry technique, analysis the correlation between this expression and clinical pathological characters of endometrial carcinoma, investigate the relationship between Survivin, CerbB-2, Ki-67 and the development of endometrial carcinoma and provide the theory basis for clinical diagnosis and treatment.Method:1 15 paraffin blocks of normal endometrium, 23 paraffin blocks of atypical hyperplasia endometrium and 52 paraffin blocks of endometrial carcinoma were collected.2 Detect the expression of Survivin, CerbB-2 and Ki-67 in endometrial carcinoma, atypical hyperplasia endometrium and normal endometrium by tissue microarray and immunohistochemistry technique.3 Analysis the data with SAS6.12. Results:1 The expression of SurvivinIn normal endometrium, the expression of Survivin is absence or part of tissues express weakly positive (40.00%). Survivin expression is extra positive in endometrial carcinoma (92.31%) and endometrial atypical hyperplasia (95.65%). Survivin expression in endometrial carcinoma and endometrial atypical hyperplasia is significant higher than normal endometrium (P<0.05); However, there is not differentiated between endometrial carcinoma and endometrial atypical hyperplasia (P>0.05).2 The expression of CerbB-2The expression of CerbB-2 in normal endometrium, endometrial atypical hyperplasia, endometrial carcinoma is respectively 33.33%, 60.87%, 76.92%. CerbB-2 expression in endometrial carcinoma and endometrial atypical hyperplasia is significant higher than normal endometrium (P<0.05); However, there is not differentiated between endometrial carcinoma and endometrial atypical hyperplasia (P>0.05).3 The expression of Ki-67The expression of Ki-67 is extra positive in endometrial carcinoma (88.46%). Ki-67 expression in endometrial carcinoma is significant higher than endometrial atypical hyperplasia (52.17%) and normal endometrium (40.00%) (P<0.05); However, there is not differentiated between endometrial atypical hyperplasia and normal endometrium (P>0.05).4 The The relationship between the expression of Survivin, CerbB-2, Ki-67 and clinical pathological featuresThe expression of Ki-67 has significant relation with histological grade (P<0.05); No significant relationship were found between the expression of Survivin, CerbB-2 and clinical pathological features, such as histological grade, myometrial invasion and operation-pathological stage (P>0.05). 5 The results of tissue microarray The tissues in 3 microarray paraffin blocks are regularity for the requirement of experiment. The results of HE stain showed the structure is good. There is no non-specificity stain and edge effect stain by immunohistochemistry. There is no differentiated between tissue microarray and routine slice method in immunohistochemiscal results (P>0.05).Conclusions:1 The expression of Survivin, CerbB-2 in endometrial carcinoma and endometrial atypical hyperplasia are all significant higher than normal endometrium. The expression of Ki-67 in endometrial carcinoma is significant higher than endometrial atypical hyperplasia and normal endometrium.2 There is no correlation about expression in Survivin, CerbB-2 and Ki-67 in endometrial carcinoma.3 The lower the histological grade of endometrial carcinoma is, the higher the positive rate of expression of Ki-67 is. Except of that, there is no relationship in Survivin, CerbB-2, Ki-67 and other clinical pathological features.4 The cell multiplication is active in the canceration of endometrial carcinoma. The abnormal expression of Survivin and CerbB-2 has played an important role in the development of endometrial carcinoma.5 The tissue microarray is a new simple technique with many advantages such as high performance, low consumption, and low experimental error. The sample is representative to reflect structural information of primitive tissue. There is no differentiated between tissue microarray and routine slice method in immunohistochemiscal results. It is practical to effectively examine massive clinical specimen with tissue microarray technique, which is rapid, convenient, economic, and accurate.