Protective Effects Of HXTML On Myocardial Ischemic Rats And On The Expression Of P-Selectin And L-Selectin

In this paper, the successful model myocardial ischemic rats with hyperlipemia and isoproterenol subcutaneously injection was developed. It is familiar to the pathologic change of coronary heart disease (CHD) hyerlipoidemia and the myocardial ischemia damage. Utilizing the model detective techniques, such as serology, radioimmunoassay , immunohistochemistry and optical microscope, etc. The paper observes the effect of the treatment of HUO XUE TONG MAI LING (HXTML), and surveys the change of myocardium zymogram, blood-fat, inflammatory factor, adhesion molecule and cadiocyte to prove the synthetic accommodation action and provide a basis for further utilization.Objective: To study the protective effects and mechanism of HXTMC on expermental myocardial ischemic rats.Methods: 50 healthy male Wister rats were randomly divided into groups namely blank group, model group, Cedocard group, HXTMLmax and HXTMLmin group, 10 rats in each group. Each rat had been recordⅡelectrocardiographic (ECG) before the experiment. Hyperlipemic forage was feeded to rats except blank group. After 12 hours abrosia on the 28th day, blood was abstracted from angular vein. After gaining statistical significance, then saline were given to Blank group and Model group at 10ml/kg weight; suspension of Cedocard were given to Cedocard group at 2.5mg/kg weight; HXTML were given to HXTMLmax and HXTMLmin group at dose of 47g/kg and 23.5g/kg weight. All of these medicines were administrated to rats at 10ml/kg weight and one time each day. The administration would continue 15 days. From the 12th day, ISO (5mg/kg·d) was subcutaneously injected into rats except Blank group to cause myocardial ischemia and the rats of Blank group were received an equal volume of saline instead of ISO for three days. After 45 minutes injecting on the 15th day, All of the rats were anaesthetized and blood was abstracted from angular vein and determined the contents of total cholesterol, triglyeried, low density lipoprotein and high density lipoprotein by CHOD-PAP enzymic method, GPO-PAP enzymic method, CAT method. Then all of the rats were to recordⅡECG. the rats were killed and the sera were collected to determine the myocardium zymogram (CK, CK-MB and LDH). Radioimmunoassay was taken to determine Tumor necrosis factor (TNF) and Interleukin-6 (IL-6). HE staining was employed to analyze the pathological damage in myocardium of heart apex and ABC staining to detect the expression of P-Selectin and L-Selectin in the myocardium.Results:1 Blood fat: The level of TC, TG and LDL-C in Model group was higher than in Blank group, The level of HDL-C in Model group was lower than in Blank group (P<0.01). Compared with Model group, the level of blood fat was better in Cedocard group, HXTMLmax and HXTMLmin group (P<0.05 or P<0.01). Compared with Cedocard group and HXTMLmin,the level of blood fat in HXTMLmax group was the best. There was statistical significance (P<0.01)). The difference of blood fat was no statistical significance between Cedocard and HXTMLmin group (P>0.01).2 ECG: Compared with the ECG before ISO injection, the ST segments and T waves were changed clinically in all groups except Blank control group, which proved that the animal model was successfully copied. Compared with Blank group, the ST segments and T waves of Model group were decreased most obviously (P<0.01). Compared with Model group, Cedocard, HXTMLmax and HXTMLmin group could alleviate the myocardial change of ECG (P<0.01), Compared with Cedocard, HXTMLmax and HXTMLmin group could alleviate the myocardial change of ECG significantly (P<0.01), but the difference between HXTMLmax and HXTMLmin group was no statistical significance (P>0.05).3 Myocardium zymogram: Compared with Blank group, the contents of creatine kinase (CK), CK-MB and lactate dehydrogenase (LDH) in Model group increased significantly (P<0.01). The results showed that zymogram of Cedocard, HXTMLmin and HXTMLmax group were significantly lower than Model group (P<0.01). The difference of Myocardium zymogram was no statistical significance in Cedocard , HXTMLmin and HXTMLmax group (P>0.05).4 TNF, IL-6: Compared with Blank group, the contents of TNF and IL-6 in Model group increased significantly (P<0.01). The results showed that TNF and IL-6 of Cedocard, HXTMLmin and HXTMLmax group were significantly lower than Model group (P<0.05 or P<0.01). The difference of TNF and IL-6 was no statistical significance in Cedocard, HXTMLmin and HXTMLmax group (P>0.05).5 Pathological changes: Obviously pathological damage had not be seen in tissue of Blank group, and in Model group, major included pathological changes of focal in most cardiac muscle, swelling and breaking in muscle fiber, vacuolar degeneration, interstitial edema and infiltration of inflammatory cells. The changes of Aspirin and HXTMLmin group were slighter than those in Model group and only included muscle fiber swelling, focal breaking, few vacuolar degeneration and infiltration of inflammatory cells. The pathological changes of HXTMLmax group were the slightest and most were infiltration of inflammatory cells.6 Expression of P-Selectin and L-Selectin: Compared with Blank group, the Expression of P-Selectin and L-Selectin in Model group increased significantly (P<0.05 or P<0.01). The results showed that the Expression of P-Selectin and L-Selectin of Cedocard, HXTMLmin and HXTMLmax group were significantly lower than Model group. The expression of P-Selectin and L-Selectin in HXTMLmax group was better than Cedocard, HXTMLmin group (P<0.01). The difference of the Expression of P-Selectin and L-Selectin was no statistical significance between Cedocard, HXTMLmin group (P>0.01)Conclusion: HXTML can alleviate the myocardial damage of rats induced by ISO and protect myocardial tissue. Furthermore, it can decrease the expression of P-Selectin and L-Selectin, which is indicated that it has some particular effects on anti-inflammation.