| Objective: Oral and maxillofacial tumor is an important composition within tumor of head and neck. In our country, squamous cell carcinoma(SCC) is the predominant classification among oral and maxillofacial malignant tumour. Although many traditional procedures such as surgical resection, radiotherapy and chemotherapy were applied to the treatment of SCC, a radical cure effects is still impossible , some lesions locating in functional area are not indications for surgical resection. Therefore ,the development of treating malignant tumor is urgent. So, how to minify operation scope, control clinical deuto-focus of infection and minute focus of infection is a problem to solve urgently. If we can use immunotherapy methods to minify operation scope,contronl clinical tumor increasing,and die in the later period may be a good message to oral cancer patient. As the most important functional APCs ,its isolation, proliferation and culture of DC are possible in vitro. Research of different kinds of DC vaccine became a hotspot as a new anti-tumor methed. by increasing animal experiments as well as initial human trials with the development of in vitro DC inducing and amplifying techniqueOver recent years, the effects of DC in tumor immunotherapy has been supported。Now DC is applied in clinical trials of B lymphoma, melanoma, prostate cancer, metastasis kidney cancer and multiplemyeloma, and satisfied effects have been achieved. In the many DC method, DCc and CTLs co-cultured with those DCs were very important ,because the effects of its in tumor immunotherapy has been supported by increasing animal experiments.In this experiment,being include from human perihecal blood monocytes with rhGM-CSF, rh(IL-4)and TNF-α,DCs were pulsed by Tca8113 cells lysates.Those DCs and T lymphocytes were implanted into the tumor-bearing mude mice.To investigate the inhibitory effect DCs on the tumor-bearing mude mice. To found the further application DCs and CTLs co-cultured with those DCs in adoptive immunotherapy of oral squamous cell cancer patient.Method: We Gathered the peripheral blood 20ml from 15 healthy volunteer, and got centrifugalized separation peripheral blood monocytes (PBMC) in common method , adjusted cell density to 1×107/ml by RPMI1640 culture fluid with 10% Fcs. Put 3ml fluid innocuiated into 6 orifice. The cells were incubated at condition of 5% CO2, 37℃for 2~3 hours. The supernatant was discarded, then cleaned board 1 times by pre-heat medium, excluded the non-adherence cell (freezed survive), got the adherence PBMC. Being induced fromhuman peripheral blood monocytes (PBMCs) with rhGM-CSF, rhIL-4 and rhTNF-α, DCs were pulsed byTca8113 cells lysates. Those DCs and T lymphocytes were co-cultured to induce specific cytotoxic Tlymphocytes(CTLs). Immunotherapywas then performed after those DCs and CTLswere implanted into thetumor-bearing nudemice.Result: DCs were induced from PBMC with multiple cytokins.Compare with the contral group, the growth of tumors was significantly inhibited in the group that had been implanted with人DCs and CTLs(P<0.05). Drawing two sets nude mice tumors growth curve , We found the control group tumors growth curve fairly steep and upgrate exetent comparatively large .On the other way ,the exeperimental groups growth curve assume to slow-moveing domntrend. When the exeperiment completed, the exeperimental groups inhibitory rate to the tumors is 26.16%, compare with the control group in paring t analysis ,the difference have statistical significance.Conclusion: Our experiments improved , the CTLs were induced by DCs that were pulsed with oral carcinoma cells lysates have significantly inhibitory effect to the growth of the tumors. DCs vaccine can be use to anti-tumors of oral carcinoma cells cancer, so it may became a new model in immunotherapy of oral cancer .
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