Utilizing The Nanosecond Pulse Technique To Improve Antigen Intracellular Delivery And Presentation To Treat Tongue Squamous Cell Carcinoma

Background: Tongue squamous cell carcinoma(TSCC)is one of the most common squamous cell carcinomas in the region of head and neck,which accounts for about 1/3 to 1/2 of oral cancer.Although the conventional surgical treatment,chemotherapy and radiotherapy can partly improve the five-year survival rate,the serious side effects often emerge after these treatments.Immunotherapy has great potential in the treatment of tongue squamous cell carcinoma because of its unique advantages.However,the efficacy of immunotherapy is limited by the efficiency of antigen phagocytosis by immune cells.Material and Methods: We extracted dendritic cells(DCs)from human peripheral blood.Utilizing a nanosecond pulsed electric field(nsPEF),we deliver the Cal-27 tumour lysate protein into DCs and then incubate the DCs with PBMCs to obtain specific T cells to kill tumour cells.The biosafety of nsPEF was evaluated by the ANNEXIN V-FITC/PI kit.The efficacy of lysate protein delivery was evaluated by flow cytometry.The antitumour efficacy was tested by CCK-8 assay.Results: In the 5 kV and 10 kV treated group,nsPEF could increase 40 % of the uptake ability of DC.In the 15 kV and 20 kV treated group,nsPEF could significantly increase 150 % and 200 % of the uptake ability of DC for tumor antigen compared with control group,respectively.The nsPEF would not seriously damage the DC in the all field strength(5 kV,10 kV,15 kV and 20 kV).In the 15 kV and 20 kV treated group,the nsPEF dramaticly enhance the killing efficiency for tumor cells in vitro.Conclusions: Utilizing nsPEF(15 kV and 20 kV)to improve the phagocytic and presenting ability of DCs could greatly activate the adaptive immune cells to enhance the immunotherapeutic effect on Cal-27,a TSCC cell line.