The Study Of O Type Blood Incompatible Transfusion

Blood loss and the consequent shock are the main reasons for war casualties. Effective transfusion is the key measure to save life in the battlefield. O type blood with titer lower than 1:128 is used as "universal blood" abroad. Before the 80's, mass O type blood incompatible infusion was carried out in China in case of emergency, but there has been relatively little report on it. The present study proceeds as follows.The method of testing antibody titer in O type blood serumTube method is the conventional method for testing antibody titer, but it is time-consuming, involving complicating operation and lots of interfering factors. The solid phase microplate method for testing red blood cell antibody is a new technology. The amended automated method uses amylaldehyde to dispose the microplate before inducing the red blood cells to combine with the board faster so that fewer cells are brushed off after repeated washing and the cell color hardly changes. The new process of full-automated sample appliance and enzyme-linked analyzer make all the steps automatic, from double-time dilution through adding samples to the result reading . So it can reduce mass labor work and human errors, further guaranteeing the operation in mass. The cost is far lower than that of the microcolum gel method. It can be used in the testing and filtration of large samples.The testing of antibody titer for O type blood serum nationwideIn order to find out whether there is significant difference in the titer of antibodies in the serum of O type blood populations in different regions in China and whether O type populations in different regions can all serve as the blood donors at war-time, the titer of IgM and IgG of anti-A(B) antibodies in O type blood serum of 480 samples from 12 cities was tested in China, suggesting no significant difference in the level of antibodies between the 11 cities as a result of the high mobility of people and inter-racial marriages. IgM antibodies titer mainly ranges from 1:8 to 1:64, with 1: 16 as the most frequent titer while the titer of IgG antibodies from 1:32 to 1:128, with 1:64 as the most frequent titer. The study of blood substance in incompatible infusion of O type bloodThe level of antibody titer tested by O type blood serum reacting with recipients' red blood cells in vitro cannot represent the fact, as it is closely related to the amount of blood substance inside the recipient's body, the actual blood volume of the recipient, transfusion speed and transfusion volume. We studied the role of blood substance in incompatible infusion of O type blood by testing the anti-A(B) titers of the same O type blood serum sample reacting with the standard red cells, special red cells and with the special red cells plus its serum. The results indicated that titers of the same O type blood serum sample reacting with the first two types of cells were not significantly different, while those with the third type was significantly different. The blood substance in the serum could effectively neutralize the antibodies in O type blood serum, so it could be inferred theoretically that the recipient can accept the blood with higher antibody titer without acute haemolytic reaction.The study of incompatible infusion of O type blood outside the bodyTo study the hemorpheology of type B recipients who receive a large dose of type O whole blood during transfusion of blood of different types, the number of red cells and the change of free hemoglobin, 7.5,10.0,13.5,20ml O type whole blood whose anti-B title is 1: 64 was added to type B whole blood collected fresh, which amounts to 800, 1200, 1600 and 2400ml blood transfused into an individual of 60 kg. This blood was placed in a 38℃hatcher, and rocked even by a rocker at the rate of 60 times per minute. Samples were collected at Oh, 2h,4h,8h,16h,and 24h and hemorpheological tests were conducted using the SA-6000 viscosimeter. Findings indicated that the groups of samples differed little in free hemoglobin and the number of red cells but differed considerably when the rate of shear was 5, 30, 200, suggesting the decline of morphotropism of cells, yet still within the range of normal. It can be concluded that O type blood whose anti-B titer is below 1: 64 can be used for type A and B at any amount.