A Study On Soluble P-/L-selectin In Childhood Asthma And Other Lower Respiratory Tract Inflammation

锘库槄Objective Bronchial asthma is the most frequent allergosis in respiratory apparatus. Childhood asthma morbidity rate is obviously rising in the cities of our country and the disease do great harm to the health of children.the pathogenesy of Bronchial asthma is complicated and not yet clear. New studies indicate that selectin play a part in the pathogenesy of Bronchial asthma.The studies about selectin and asthma almost base on animal model or in vitro study and only the study on one of selectins and asthma can be seen. To investigate the significance of soluble P-selectin and L-selectin in the pathogenesy, testing laboratory diagnosis,differential diagnosis of childhood asthma, we mensured the serum level of soluble P-selectin and L-selectin inchildhood asthma, bronchiolitis, asthmatoid bronchitis and bronchopneumonia.鈽匨ethods The serum level of soluble P-selectin and L-selectin were detected in 42 cases of acute asthma attacks of child, 18 children with stable asthma, 30 cases of bronchiolitis, 24 cases of asthmatoid bronchitis, 30 cases of bronchopeumonia and 30 healthy children by using a sandwich ELISA procedure.The analysis of variance, Fisher's least significant test and correlation analysis were carried out for the results of determination using SPSS11．5 software package.鈽匯esults鈽?. about P-selectin:鈶燭he serum level of soluble P-selectin in acute asthma attacks of child was significantly higher than those in children with stable asthma and healthy children, P<0．01; while no significant difference between the latter two groups, P>0．05．鈶he serum level of soluble P-selectin between acute asthma attacks of child and children with bronchiolitis was no significant difference, P>0．05; while that in children with stable asthma was significantly lower than that in children with bronchiolitis, P<0．01．③The serum level of soluble P-selectin in acute asthma attacks of child was significantly higher than that in children with asthmatoid bronchitis,P<0．01;while that of children with stable asthma was significantly lower than that in children with asthmatoid bronchitis , P<0．01．④The serum level of soluble P-selectin in acute asthma attacks of child was significantly higher than that in children with bronchopneumonia, P<0．01;while that of children with stable asthma was significantly lower than that in children with bronchopneumonia, P<0．01．⑤The serum level of soluble P-selectin in children with bronchiolitis was significantly higher than those in children with asthmatoid bronchitis ,with bronchopeumonia and healthy children, P<0．01; while no significant difference between asthmatoid bronchitis and brochopeumonia, P>0．05．2．about L-selectin:①The serum level of soluble L-selectin in acute asthma attacks of child and children with stable asthma was significantly higher than that in healthy children, P<0．01; while no significant difference between the former two groups, P>0．05．②The serum level of soluble L-selectin among acute asthma attacks of child and children with stable asthma and children with bronchiolitis was no significant difference, P>0．05．③The serum level of soluble L-selectin in acute asthma attacks of child and children with stable asthma was significantly higher than that in children with asthmatoid bronchitis , P<0．01．④The serum level of soluble L-selectin in acute asthma attacks of child and children with stable asthma was significantly higher than that in children with bronchopneumonia, P<0．01．⑤The serum level of soluble L-selectin in children with bronchiolitis was significantly higher than those in children with asthmatoid bronchitis ,with bronchopeumonia and healthy children, P<0．01; while no significant difference among the latter three groups, P> 0．05．3．There is positive relation between the serum level of soluble P-selectin and L-selectin in all groups, r=0．591, P<0．01．Conclusions①There is higher serum level of soluble P-selectin and L-selectin in acute asthma attacks of child. The serum level of soluble P-selectin can reflect Severity of asthma, while L-selectin can reflect air hyperreactivity and further distinguish body constitution of children with stable asthma and healthy children.we can evaluate asthma condition and guide treatment by determining the serum level of soluble P-selectin and L-selectin.②There is higher serum level of soluble P-selectin and L-selectin in acute asthma attacks of child and bronchiolitis. We can presume that the two diseases have same or similar pathogenesy and air hyperreactivity.we can further investigate whether or not the serum level of soluble P-selectin and L-selectin can predict prognosis of bronchiolitis.③The serum level of soluble P-selectin and L-selectin in children with asthmatoid bronchitis was significantly lower than that in acute asthma attacks of child. This presume the two diseases have different pathogenesy and severity and should not be treated as one. We should further investigate whether or not the serum level of soluble P-selectin and L-selectin can predict prognosis of asthmatoid bronchitis.④P-selectin and L-selectin may become new target of asthma treatment and provide new thought and theory for prevention and cure of asthma.⑤There is positive relation between the serum level of soluble P-selectin and L-selectin.