| Objective To observe the inhibitory effect of NAPP on Hepatitis B virus both in vitro and in vivo. It may lead a theoretical foundation on developing of a new kind of anti-virus medicine.Methods The cell 2.2.15 line was taken as cell model. The different concentration of NAPP water solution were respectively added into cell culture fluid of 2.2.15 cell strain. The cell toxicity of NAPP was determined with MTT method and the inhibitory effect of NAPP on HBsAg and HBeAg secreted by 2.2.15 cells was evaluated on the ninth day with ELISA method. Real-time fluorescence quantitative PCR was used for detection of HBV-DNA in the culture medium. At same time, we took lamivudine as a control to evaluate the effect on anti-HBV. Duck model of congenital infection of HBV was used as animal model. The ducks were divided into three groups at random and the each group ducks was given the drug at the 20mg/(kg·d) for 10 successive days. The dynamic changes of serum duck HBV-DNA titer was detected by dot-hybridization test before treatment and after treatment. Fresh liver tissue ware sheared. The pathological changes of the duck liver were also examined by HE staining.Results In the cell model, NAPP had no obvious cell toxicity at the concentration of 1mg/ml. NAPP had obvious restrain effect on HBsAg and HBeAg at the different concentration and had a dose-dependent. The therapeutic index of NAPP was 188.7 for HBsAg and 64.5 for HBeAg. In the animal model, the optic density value of DHBV-DNA in ducks serum got down markedly after giving medication with NAPP for 5 days and 10 days, there were remarkable difference (T5, P<0.05; T10, P<0.01). Compared with lamivudine control group, there was no significant difference (P>0.05). Spotty of hepatocyte in NAPP group was improved more obviously than that in model group.Conclusion NAPP exerts a certain inhibitory effect on Hepatitis B Virus both in vitro and in vivo. NAPP can protect duck liver against inflammation.
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