Effects Of GM6001 On Expression Of Matrix Metalloproteinases In Cornea Tissue Of Fungal Keratitis

Objective Investigating the expression of matrix metalloproteinase-1 (MMP-1) infungal keratitis in order to study the lesion mechanisms of MMP-1 to cornea and study theeffect of it's selective inhibitor GM6001 on the expression of MMP-1 in fungal keratitis ofNew Zealand white rabbit.Materials and methods 75 New Zealand white rabbits were derided into 3 groups atrandom, including group A ,B and C. Group A was control group, including 5 corneas.Group B was fungal keratitis group, including 35 corneas. Group C was fungal keratitisgroup treated by GM6001, including 35 corneas. Aspergillus were inoculated in corneas ofB,C groups. After inoculating, the corneas of group C were dropped with 400μg/mlGM6001 at once.After inoculating, the expression of MMP-1 in 35 fungal keratitis (at 12h,24h,4d,7d,14d,21d,28d) and 35 treated corneas was detected by semi-quantitativereverse transcription-polymerase chain reaction (RT-PCR) and the corneas were given HEstaining and light microscope study.Results 1. Histopathological examination: Fungal keratitis was a kind of suppurativeinflammation, including the invasion of fungal hyphae and conidia and the infusion ofneutrocytes in large quantity. 2. RT-PCR results: (1)There was no expression of MMP-1in control and 12h' corneas. 24h after inoculating, MMP-1 mRNA was upregulated and thedifference between different phases was significant (P＜0.01). (2) 24h after inoculating,the expression of MMP-1 in Group C was lower obviously than that in Group B at everyphase, and the difference was significant (P＜0.01).Conclusion 1.These results suggest that the abnormal expression of MMP-1 plays animportant role in the occurrence and development of fungal keratitis. The high level ofMMP-1may be an objective evidence of diagnosis. 2.GM6001can inhibit the expression ofMMP-1 in fungal keratitis, which may become the basement of drug therapy andprevention of fungal keratitis.