| AIM We established the irradiation damage models of thymocytes in vitro to study the protectiveeffects and mechanism of PCF (polypeptides from Chlamys farreri) on thymocytes from the micedamaged by UV irradiation (6.16mJ/cm~2). MATHODS The viability of thymocytes were determinedby MTT; The apoptosis on thymocytes were tested using flow cytometry (FCM); The free calcium,mitochondria membrane potential and the rate of apoptosis on thymocytes were testedusing flow cytometry (FCM); The expressions of Bax and Bcl-2 proteins were examinedby immunocytochemical technica; flow cytometry was used in examining Caspase-3 production; Geneexpression profile was identified by cDNA microarrays in thymocytes exposed to UV and pretreatedwith PCF RESULTS The results indicated that PCF could significantly increase the viability ofthymocytes and decreased the rate of apoptosis in cells(P＜0.01); PCF decreased the amounts of ROS,MDA (P＜0.05); It also raised the expression of Bcl-2 gene and decreased the expression of Bax (P＜0.05), that means PCF could block the UV damage of mitochondria. The result of flow cytometryreveal that (0.125-0.5%) PCF dose dependent depress thymocytes Caspase-3 contents (P＜0.05). Theresult of gene array indicate that regulating apoptotic gene UCP-2,VDCA-1 Bcl-2 and Caspase-3,8were related to the irradiation of UV. and the protective effects of PCE CONCLUSION PCF had theprotective effects on damages of thymocytes caused by the irradiation of UV. The mechanisms mightbe related to inhibiting of the amount of ROS, the regulation of mitochondrium apoptosis signaltransduction. And inhibition the amount of Caspase-3.
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