An Experimental Study On The Effects Of TPP Preventing And Treating As In Rabbits

Objective: An experimental study on the effects of Turbid-Diarrhoe a and Phlegm-Eliminating Prescription(TPP) to promote blood circulati on on smooth muscle cell proliferation and apoptosis in vascular wall after vascular intima was injured of Atherosclerosis(AS)in rabbits an d to investigate the possible action mechanisms.Method: Thirty-two healthy male or femaleNew Zealand big-earwhi te rabbits (Weight:2.4-2.6kg)were randomly divided into four groups, t hey are normalgroup, model group, control groupand treatment group, Ever y group has eight animal. The rabbit model of As was established af the r being fedhigh fat diet for four weeks. Normal group was fed with reg ular rabbit deit Other groups were fed with high fatdiet throughout t he experiment. First week , The trwament group was given TPP through f illing stomach one time every day for six weeks. The control group wa s given west medicine. And the normal group and the model group were given water instead. Before injured vascular intima of the rabbit whi ch were feded by fat diet for 4 weeks except normal group. At the sixt h week, serum total cholesterol(TC),triglycerides(TG),low desity lipo protein cbolesterol(LDL-C), high desity lipoprotein cholesterol(HDLC), were measured. At the same time , barbitone narcosis and the local vasculars were collected for PDGF-B,c-myc and IGF-1 levels measureme nt. at the end of the study, the rabbits were narcosisdeand pathologic changes ofvessel intima were observed under light microscopy and elec tronic microscopy.Result: PDGF-B,c-myc,IGF-1 and serum lipid in AS in control gro up increased significantly compared with the normal group(P＜0.05). PD GF-B,c-myc,IGF-1 and serum lipids in Chinese traditional medicine g roup and western medicine group decreased significantly compared with the model group. Conclusion: TPP can stop the beginning and development of AS;after vascular intima injured, PDGF-B,e-myc,and IGF-1 increased. TPP promot e blood circulation can promote smooth muscle cell proliferation and restrain it apoptosis.