Expression And Correlation Of SDF-1 And MMP-9 In Human Laryngeal Squamous Cell Carcinoma Tissues

Objectives: Many malignant tumor cells can secrete chemokines,which interact with tumor cells,study on chemokines has become one of the hot fields of cancer research;stromal cell derived factor is a class of small molecule protein chemotaxis,plays an important role in the differentiation and metastasis of tumor occurrence and development.Matrix metalloproteinases?MMPs?are a group with proteolytic function of zinc containing protein hydrolase family,are responsible for the degradation of extracellular matrix components in normaltissue remodeling and repair process,the excessive or inappropriate expression may be an important mechanism of invasion and metastasis of carcinoma.This article through the detection of stromal cell derived factor 1?SDF-1?and matrix metalloproteinase 9?MMP-9?expression in laryngeal squamous cell carcinoma and evaluate the roles of SDF-1 and MMP-9 expression in laryngeal squamous cell carcinoma and clinical significance.Methods: A retrospective study was conducted.42 cases paraffin-embedded specimens obtained from surgical resection with complete clinical data,and another 19 cases of adjacent laryngeal laryngeal mucosa were collected as controls from the Department of Otorhinolaryngology Head and Neck Surgery of the Neijiang First People's Hospital from February 2006 to February 2011.Survival and recurrence of laryngeal cancer patients were followed,as well as the clinical and pathological data.The immunohistochemical method?SP?was used to detect the expression of SDF-1 and MMP-9 in 42 cases of laryngeal carcinoma and 19 cases of adjacent laryngeal mucosa.The relation between the expression of SDF-1,MMP-9 and clinical pathological features and prognosis of patients with laryngeal squamous cell carcinoma were analyzed,as well as clinical data,such as age,sex,duration,pathology,TNM stage,clinical stage and follow-up results.Results:?1?The positive rate of SDF-1 in the laryngeal squamous cell carcinomas was 52.4%,which was higher than that in the adjacent negative laryngeal mucosa?21.1%??x²=5.250,P=0.022?.The positive rate of MMP-9 in the laryngeal squamous cell carcinomas was 59.5%,which was higher than that in the adjacent laryngeal mucosa?26.3%??x²= 5.772,P=0.016?;?2?There was no significant difference in the expression of SDF-1 and MMP-9 in laryngeal squamous cell carcinoma?LSCC?between different age,gender,duration of disease and smoking habit?all P > 0.05?;?3?The positive expression rate of SDF-1 in laryngeal squamous cell carcinomas with T_1-2 stage was 38.7%,which was lower than that with T_3-4 stage?90.9%??x²=8.869,P=0.003?.The positive expression rate of MMP-9 in laryngeal squamous cell carcinomas with T_1-2 stage was 41.9%,which was statistically different from that with T_3-4 stage?81.8%??x²=5.177,P=0.023?;?4?The positive rate of SDF-1 was 33.3% in stage N0 patients,which was lower than that in N1-3 patients?100.0%??x²=15.273,P=0.000?.The positive rate of MMP-9 was 43.3% in patients without metastasis?N0?,which was lower than that in patients with metastasis?N1-3??83.3%??x²=5.536,P=0.019?;?5?The positive expression rate of SDF-1 was 30.0% in patients with stage?-?,which was lower than that with stage ?-??72.7%??x²=7.668,P=0.006?.The positive rate of MMP-9 in patients with stage?-?was 40.0% and 72.7% with stage ?-?,the difference was statistically different?x²=4.582,P=0.032?;?6?The positive rate of SDF-1 was 43.5% in the well differentiated laryngeal squamous cell carcinomas and 63.2% in the moderately and poorly differentiated laryngeal squamous cell carcinomas.There was no significant difference between these two groups?x²=1.616,P=0.204?.The positive rate of MMP-9 in patients with well differentiated laryngeal squamous cell carcinoma was 34.8%,which was lower than that in moderately and poorly differentiated patients?78.9%??x²=8.192,P=0.004?;?7?The positive rate of SDF-1 was 41.2% in the glottic laryngeal squamous cell carcinomas and 62.5% in the non-glottic laryngeal carcinomas.There was no significant difference between these two groups?x²=0.484,P=0.487?.The positive rate of MMP-9 in the glottic laryngeal squamous cell carcinomas was 47.1%,and 87.5% in the non-glottic laryngeal carcinomas,the difference was not statistically different?x²=2.799,P=0.094?;?8?There were 17 cases in laryngeal squamous cell carcinoma patients positively expressed both SDF-1 and MMP-9,and 12 cases negatively expressed both SDF-1 and MMP-9.SDF-1 and MMP-9 expressed in laryngeal squamous cell carcinomas were positively correlated,correlation index r=0.379,P=0.014;?9?The overall survival rate of SDF-1 positive expression patients was 45.0% and 81.8% of SDF-1 negative expression patients,the difference between them were significantly different?x²=4.334,P=0.037?.The disease-free survival rate of SDF-1 positive expression patients was 61.1% and 85.7% of SDF-1 negative expression patients?x²=5.901,P=0.015?.The overall survival rate of patients with MMP-9 positive expression was 36.8%,which was lower than that of negative patients?82.6%??x²=4.023,P=0.045?.The disease-free survival rate of patients with positive MMP-9 expression was 56.2%,which was lower than that of negative patients?87.5%??x²=3.880,P=0.048?;?10?Neither of SDF-1 and MMP-9 expressed in laryngeal squamous cell carcinomas was an independent risk factor for the prognosis of patients,which was analyzed by COX risk regression model.Conclusions:?1?SDF-1 was highly expressed in laryngeal squamous cell carcinomas,but not related to pathological differentiation,suggesting that it was related to the tumorigenesis of laryngeal carcinoma.MMP-9 was highly expressed in laryngeal squamous cell carcinomas and had a higher expression rate in moderately and poorly differentiated tissues,suggesting that it was associated with the tumorigenesis and malignant differentiation of laryngeal squamous cell carcinoma;?2?The expression of SDF-1 and MMP-9 in laryngeal squamous cell carcinomas was not related to age,gender,duration of disease and smoking habit;?3?The expression of SDF-1 and MMP-9 in laryngeal squamous cell carcinomas was negatively correlated with T staging and clinical staging,suggesting that both of them may be related to the progress of laryngeal carcinoma;?4?The expression of SDF-1 and MMP-9 in laryngeal squamous cell carcinoma of patients with cervical lymph node metastasis was higher than that without metastasis,suggesting that both of them were associated with cervical lymph node metastasis;?5?The expression of SDF-1 and MMP-9 in laryngeal squamous cell carcinomas was not related to the clinical classification;?6?SDF-1 and MMP-9 in laryngeal squamous cell carcinoma were positively correlated,suggesting that they may have synergistic effect in the tumorigenesis and development of laryngeal carcinoma;?7?The expression of SDF-1 and MMP-9 in laryngeal squamous cell carcinoma were negatively correlated with disease-free survival rate and overall survival rate;?8?The value of SDF-1 and MMP-9 expressed in laryngeal squamous cell carcinoma in evaluating the prognosis of laryngeal cancer patients needs further study.