The Expression Of AQP-1 CTGF And VEGF In Nasal Polyps

Objective: To elucidate the correlation of Aquaporin-1 (AQP-1), connective tissue growth factor (CTGF), and vascular endothelial growth factor (VEGF) with nasal polyps. Nasal polyps is the common disease in otorhinolaryngology surgery, the symptoms include nose stuffing and loss of odor . Nasal membrane edema, blood vessel proliferation, gland proliferation, and submucous fibrocyte hyperplasia are the main pathological change of nasal polyps.The most accepted theory concerning the formation of nasal polyps is multiple etiology .AQP-1 is relate to the development of edema , CTGF triggers the fibrosis, and VEGF is regard as the important factor of the vessel growth and vascular permeability. Reports on the correlation between these three proteins and nasal polyps are scarce. Our aimed to explore their link to the development of nasal polyps, and further more their internal correlation.Methods : Samples were collected from 11 patients of the nasal polyps and 10 patients of spur of nasal septum who were operated.The nasal polyps samples and normal nasal mucus membrane at the end of the middle turbinate were taken during the functional endoscopic nasal surgeryoperation .The samples are fixed with 4% formaldehyde and embedded in paraffin.The slices were stained with anti-human AQP-1,anti-human VEGF and anti-human CTGF through the method of immunohistochemistry. The percentage of positive cells and intensity were calculated. The data was proceeded using of SAS software. The cellular population specific distribution of these three proteins in the tissues were compared, and sub-cellular localization of each protein was evaluated . Measure the thickness of the fundus layer of nasal mucus membrane under the high eyeshot ,then evalue if there are different between the two groups by analyse of SAS software.Result :①The masculine rate of AQP-1 what appeared on the nasal polyps'membrane epidermis cell is lower than that of the normal nasal mucus membrane's cell(P=0.0292<0.05), The gland under the mucous membrane have no evident distinction between the two groups in the masculine rate .②The intensity of the nasal polyps'mucous membrane epidermis cell is lower than the normal tissue too(P=0.0094<0.05). The gland under the mucous membrane have no evident distinction between the two groups in the intensity. The intensity of AQP-1 in the blood vessel endodermis under the mucous membrane has no distinction too .③There has no evident distinction between the to groups in the nasal mucous membrane epidermis'CTGF masculine rate .But in the gland cell,the CTGF masculine rate of nasal polyps'group is higher than the normal group (P=0.0246<0.05).④The intensity of CTGF in mucuse member epidermis'group is higher than nasal polyps' group (P=0.0451<0.05), but the intensity of CTGF in mucuse member epidermis'group have no distinction between two groups . The intensity of CTGF in blood vessel endodermis of nasal polyp's group is higher than the nomal group(P=0.0486<0.05).⑤There has no evident distinction between the two groups either in nasal mucous membrane epidermis'VEGF masculine rate or in gland's rate.⑥The intensity of VEGF in nasal mucous membrane epidermis , gland under the mucous membrane and the blood vessel endodermis have no distinction .Conclusion :AQP-1 may have relativity with the form of nasal polyp , Water egest from nasal mucous membrane may be the important reason of nasal polyp's formation .The intensity of CTGF's expression in mucous membrane increase may be the important reason of connective tissue hyperplasia in nasal polyps..VEGF expression have not evident difference between nasal polyp and normal nasal mucous membrane.