| With the expansion of industrial and agricultural production, more and more toxic substances are expelled to the surroundings, introduced into human bodies. Many of these compounds are carcinogenic, teratognic and mutagenic to human. Drinking water safety has posed a series of life risks for people, especially detection of Enviromental Harmon (EHs) were highly regarded. Analytical techniques allow for the determination of EHs residues in drinking water samples.However, they require time-consumed procedure for sample preparation before analyzing so as to remove or eliminate the effects from matrices.In this thesis, we concenteated ourselves on development of simple, rapid and accurate liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for analysis of Enviromental Harmon residues in drinking water.This dissertation consists of three parts.1,Solid phase extraction (SPE) for the sample preparationA variety of factors, which effected on the recovery of the nine EHs in drinking water, have been investigated such as SPE cartridge, washing solvents, elution solvents. The results give good recoveries of water samples. The best conditions were recived: nine EHs can be enriched by using a C18 cartridge, washing solvent was methanol-water(10:90,V/V) and the analytes were eluted by 8mL acetone. The recoveries of analytes are more than 80%.2,Three methods of LC-MS/MS were developed for determination of nine EHs. Three methods were compared and rapid analysis methods were established for any detection demands.(1) An HPLC-MS/MS method was developed for determination nine EHs from drinking water. Chromatogram column: Zorbax C18, 4.6×250mm,5μm;a linear gradient profile with a water(A)-methanol(B) eluent: from 0～18min A:B was 50:50(V/V); from 18～35min A:B was 20:80(V/V); flow rate:0.6mL/min; time subsection mode of MS (mass spectrometry) conditions were setted. Quantitative analysis of nine EHs was finished in 55min. The linear range for these EHs was from 1 to 100μg/L. (2) The second method of HPLC-MS/MS was developed. Chromatogram column: Zorbax C18, 4.6×250mm,5μm; a water(A)-methanol(B) eluent was 5:95 (V/V); flow rate:0.6mL/min; time running on mode of multi-reactions monitoring (MRM) scan. Eignht EHs expcet BPA were determined by positive mode. Quantitative analysis for the eight EHs was finished in 7min and achieved rapid analysis.(3) For diferent detection purpose, the method of HPLC-MS/MS was optimized by not using C18 chromatogram column. A water(A)-methanol(B) eluent was 50:50(V/V); flow rate:0.2mL/min; specifically for one kinds of nine EHs. Every analyte needn't be separated by HPLC, and can use powerful detecting function of mass spectrum. This method only takes 7mins for these analytes.3,Diferent water samples from four waterworks and different seasons in Nanchang city were determined by using these methods. The results show that most of pesticide EHs were detected and their contents changes with the season diversity; aldicarb,simazine and carbaryl were not detected in Ganjiang and tap water in Nanchang; But BPA and DBP were ubiquity, the concentration of DBP was 28.9ng/L in summer. The main reason was the widely use of plastic products. Although concentration levels of them were under the water quality standard for drinking water (GB 5749-2006), their harmfulness should be highly considered because this kind of products are increasingly used.The three detection methods of HPLC-MS/MS were rapid, sensitive and credibile.Can provide simple, rapid method for monitoring EHs in drinking water.
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