Analysis Of Radix Isatidis Injection By Capillary Electrophoresis

As a separation and analytic technology, CE is efficient, quickly, small bulk of sample, little solvent consumption, powerful antipollution, a high degree of automation, and has various models of separation, shows great advantages and potential in analysis of traditional Chinese medicine. Radix Isatidis Injection is used as one of anti-infective agents clinically. Currently, there is no unified national criterion for quality control. Different manufacturers'products differ greatly in their composition and content. In order to effectively control the quality of Radix Isatidis Injection set up a simple and stable method is imperative.This subject is to analyze the primary amino acids in Radix Isatidis Injection based on capillary zone electrophoresis (CZE) and coordination interaction capillary electrophoresis with direct UV detection. The effect of operating conditions was investigated respectively. So that it can simply and stably analyze muti-compenent in Radix Isatidis Injection synchronously. The innovation is just here. The main topics of the research and results:First, comprehensive study the operating conditions by CZE , under the optimum conditions, the right Radix Isatidis Injection multiple active ingredients had been analyzed, added the criterion five organic acids (syringic acid, salicylic acid, benzoic acid, anthranilic acid, caffeic acid) in Radix Isatidis Injection for qualitative analysis. Experimental results showed that the peak of the five components of the organic acids of Radix Isatidis Injection was low, namely, the content of those organic acids were very little, especially syringic acid. The time of salicylic acid, benzoic acid, anthranilic acid is most near. So, I think that, syringic acid, salicylic acid, benzoic acid, anthranilic acid detected by CZE as quality control indicators of Radix Isatidis Injection is not appropriate.Under the optimization of the above, the components in Radix Isatidis Injection from the three manufacturers were analyzed by CZE. Comparing the different CZE spectra, we found that the peak number, peak time, peak position, and the peak area of products from different manufacturers is great different. Account for that the clinical use Radix Isatidis Injection of different manufacturers, the contents and component are marked differences. There is an urgent need to more establish, strict and effective separation method for Radix Isatidis Injection The issue has also established a coordination role on the capillary electrophoresis methods, the use of non-derivative direct UV detection, Radix Isatidis injection of the main components of the amino acid analysis, By examining CICE running buffer distribution centers ion concentrations of acidity, pH buffer, the operating voltage, Sampling time and capillary diameter and many other factors, such separation in the mode of separation and detection. And the optimal conditions, the right Radix Isatidis injection of the two amino acids (Arg, Pro) for qualitative and quantitative analysis.The evaluation of antibiotic activity of Radix Isatidis Injection was also performed by disc agar diffusion (K-B method) and tube dilution method. As a result, Radix Isatidis Injection has no obvious antibiotic activity against seven bacteria include staphylococcus aureus, streptococcus beta-haem, escherichia coli, proteus vulgaris, shigella dysenteriae, salmonella typhi, pseudomonas aeruginosa. The injection sample was prepared by water extracting ethanol precipiting process. The main active compositions of the injection was destroyed in the preparation process, result in the weak of antibotic activity in evidence. It indicates that the extraction process of Radix Isatidis injection must be improved and optimized immediately.