| Fomes fomentarius is a medical higher fungi. Chemical constituents of Fomes fomentarius were studied the chemical constituents, medical action, exploitation and biological characteristic of Fomes fomentarius were reviewed.Through preliminary systematic test on Fomes fomentarius, the result indicated that it contains phenols, organic acids, saccharides, polysaccharides and its glycosides, lactone, coumarinic lactone and its glycosides, steroids, triterpenoids, anthraquinones and its glycosides. And it perhaps have vegetable tannins, flavonoids and its glycosides.The anticancer activities in vitro of petroleum ether extract, chloroform extract, ethyal acetate extract and n-butanol extract were screened with MTT method. The result indicated that different extracts had some inhibitory effects against hela cells, and showed significant does-dependent response. Chloroform extract and ethyal acetate extract strongly inhibited cell proliferation of hela cells at the rate of 77.37% and 69.63% when the Concentration is 1000μg·ml-1;n-butanol extract and petroleum ether extract weakly inhibited at the rate of 54.35% and 26.31%. IC50 value of petroleum ether extract, chloroform extract, ethyal acetate extract and n-butanol extract were 303.083μg·ml-1,6939.134μg·ml-1,337.954μg·ml-1574.715μg·ml-1. So the chloroform extract and ethyal acetate extract were the principal anticancer extracts from Fomes fomentarius.Inhibition of different extracts against two tested fungi was studied by growth rate method. The result showed that n-butanol extract inhibited Alternaria longipes and Fusarium solani at the rate of 68.32 and 56.41%.Five compounds were isolated from the chloroform extract of Fomes fomentarius by the methods of solvent extraction, thin chromatography, silica gel chromatography, sephadex LH-20, recrystallzation and so on. There of them were identified by methods of 1H–NMR, 13C-NMR and MS asβ-sitosterol (â… ) , [5α,8α-epidioxy-(22E,24R)-ergosta-6,22-dien-3β-ol] (â…¡) and ergosta-7,22-dien-3-one (â…£).
|
PDF Full Text Request