A Study On Biology And Evaluation Of Germplasm Of Trolliu Chinensis Bunge

Trollius chinensis is a kind of common use traditional Chinese medicine, also being medicine of mongoloid in common use. The exploitation to T. chinensis depend on wild resource mainly in China.With the fact that the raw material needs amounts rising gradually, wild resource and their reserves had sudden dropped.The germplasm resource studying method Analytical Chemistry and resource wielding Biology learn method to germplasm evaluation of T. chinensis is carried out on studies. The index centering on multicolored output and active constituent contents, is to spend the headquarter quantity characteristic combining with external Biology characteristic difference and inherent difference is to inherit diversity as well as the molecule mark technology is analytical , quality resource synthesizes exploration germ plasm evaluation of T. chinensis to appraise system, the source quantity and mass problem can be resolved, so it can construct Chinese medicinal crop serve.Study main result originally as follows:1.By the fact that the meteorological factor acquisition and analysis, have grasped T. chinensis climate characteristic.2.The pollen can sprout in cane sugar solution, the rates sprouting are big comparatively in 5%～10% cane sugar solution, overtop without exception in water sprouting rate. Rates difference T. chinensis first florescence and florescence is not big , the bud time pollen hardly can sprout.3.The pollination by 3 years has been tested , has affirmed T. chinensis belonging to common allogamy plant, has provided the theory basis to T. chinensis crossbreeding.4.The output having been in progress to T. chinensis 5 species quality is appraised, germplasm C, germplasm D, germplasm B and germplasm X flower output are consistent without G, kind of quality G output is lowest. 5 kind of quality Vitexin contents are germplasm X＞germplasm C＞germ- plasm G＞germplasm B＞germplasm D in order, germ plasm X contents are maximal.5.From flower quantitative characteristics investigation and statistic analysis , having ascertained fresh weight , dry weight, flower diameter and petal number is that main part affecting output's appraises germ plasm of T. chinensis.6.Have made use of AFLP molecule mark technology, have analysed T. chinensis inheriting diversity, various quality is many state place percentage (PPL) By : D (51.15%)＞X＞G＞C＞B (40.11%), germplasm D the state place counts a percentage is most highly, germplasm B is minimum.