Effect Of Kang Ai Zhusheye On Antiradiation In Mice And Mechanism Observation

Objective: research radiation damage mechanism and in this foundation discuss Whether there is protective function does the the Kang Ai injection to damage to mouse's subacute emission, if has, further discusses its protective function the mechanism. Method: 1st, 180 mice divide into 0Gy, 2Gy and 8Gy 3 monitoring team stochastically, three intervention groups respectively are 20,60,100.Uses X beam whole body irradiation separately 1 time,Accepts 2Gy and the 8Gy radiation mouse separately divides into two groups again, each is 30 and 50.The experimental group injects with the Kang Ai injection abdominal cavity, control group physiological saline as tales dose abdominal cavity injection, method with front. After the illumination 4h,3d and 14d, extract the mouse eyeball blood separately, with flows the type cell meter to carry on circumference blood T lymphocyte subgroup CD₄⁺ and the CD₈⁺ determination. Simultaneously kills the mouse to take its thymus and the spleen exactly, after the paraffin wax embedding, observes under the light microscope and the electron microscope change. Takes out the fresh thymus and the spleen in addition grinds separately, makes the cell to hang the fluid, with flows the type cell instrumental measurement to decide the cell to perish weakly rate. The partial cells hang the fluid with to gather the lysine processing slide smear to prepare the immunity group to use,to measure the mouse thymus lymphocyte perishes weakly the correlation protein Bcl-2 protein and the Bax protein masculine cell rate. 2. the experimental the mouse, divides into 4 groups stochastically each group of 20.Predose each group of mice draw blood make the white blood cell counting. Kang Ai group and the radiation control group shines by the identical dosage one time. After the illumination Kang Ai group the altogether three groups carry on the different dosage Kang Ai injection separately the intervention, after five days draw blood make the while blood cell counting, simultaneously observe the mouse average survival time or the 30d survival percentage. The radiation control group injects with the physiological saline abdominal cavity. ResuIts:Under the light microscope changes: After the radiation the mouse thymus cerebral cortex lymphocyte drops suddenly, third days fall to the lowest level, the 14th day thymus structure starts to restore. Electron microscope: Radiates the latter 4 hour mouse thymus lymphocyte to appear the change which the model the cell perishes weakly. After the different dosage X illumination 4h,the thymus and the spleen cell perish weakly rate namely the obvious ascension, three weather reach the peak, 14 days have the restoration slightly.The circumference blood lymphocyte subgroup quantity change, in which cell to radiates by CD₈⁺ the T sensitively, the drop scope is biggest.The Kang ai medicine group and the radiation control group compares, it to accepts 2Gy and the 8Gy radiation group thymocite accent perishes rate has the obvious inhibitory action (P<0.05). To the mouse which accepts the 2Gy radiation, the Bcl-2 protein expression and the Bax protein expression radiates 24h and the 3 days Kang Ai injection group and the radiation control group compares (P<0.05) has the difference. The Kang Ai injection to the mouse which accepts the 2Gy radiation, may promote the Bcl-2 protein expression,suppresses the Bax protein the expression; Radiates the 14th day,the radiation control group and the Kang Ai injection group compares (P>0.05), Bcl-2 and the Bax protein expression tends to close.To accepts the 8Gy radiation mouse, after radiates 24h, 3d, 14d the Kang Ai injection group and the radiation control group quite is even is (P>0.05), the Kang Ai injection to accepts 8Gy the radiation mouse Bcl-2 protein and the Bax protein expression, affects not obviously. The Kang Ai injection may obviously lengthen the 7.5Gy illumination mouse average survival time,enhances the 30d survival percentage and the white blood cell number. The Kang Ai injection group and the radiation control group compares, is shone mouse WBC to elevate obviously (P<0.05). Conclusion: Kang Ai injection can obviously suppress the mouse and the 8Gyradiation mouse's thymus, the spleen cell which accepts the 2Gy radiation perishes weakly rate. The Kang Ai injection to the mouse which accepts the 2Gy radiation, radiates 24h and the 3d day may promote the Bcl-2 protein expression,suppresses the Bax protein the expression. Radiates 14th day this kind of function not to be remarkable. The Kang Ai injection to the mouse which accepts the 8Gy radiation, after radiates 24h, 3d, 14d the Bcl-2 protein and the Bax protein expression affects not obviously. The Kang Ai injection suppression cell perishes rate is weakly through promotes the Bcl-2 protein expression, suppresses the Bax protein the expression to regulate. The Kang Ai injection to accepts the radiation the mouse immunity function to have certain protective function. The Kang Ai injection has the emission protective function to the mouse.