The Research Of The Mechanism That E The Technique Oil Makes Person Ovarian Cancer Cell SKOV3 Perishes Weakly

ObjectObserve the effects of suppressing the multiplication of person ovarian cancer cell line SKOV3 under diffirent density of e the technique oil inoculation fluid. Study the possible mechanism and action principle.MethodsUse MMT to test after deal with the person ovarian cancer cell SKOV3 under diffirent density(120ug/ml,240ug/ml,480ug/ml,960ug/ml) of e the technique oil, the multiplication situation of the cells. Calculate the suppress rate(%)=(1- experimental group extinction value/control group extinction value)×100%. Repeat the experiment steps three times. Take the average value of the three experiment. Take the medicine density as the abscissa and take the cell suppress rate as Y-coordinate.Use optical microscope to observe the Morphological change of cells before and after medicine. The experiment divides into the blank control group and the experimental group. The experimental group establishes a branch four densities:120ug/ml,240ug/ml,480ug/ml,960ug/ml e the technique oil. Cultivate them 48 hours after medicine and collect cells, fixed and HE dyeing.Immunity group reduction(according to reagent box instruction booklet operation) exam the cell Bcl-2 and Bax two kinds of protein expression change situation of control group and experimental group.Results1. E the technique oil has inhibitory action to the multiplication of person ovarian cancer cell SKOV3. It's intensity will increase with the medicine response time and density increase. 2. E the technique oil perishes weakly to the ovarian cancer SKOV3 cell the influence:Blank control group:The cell assumes spindle-shaped or the polygon, the amnion and the nucleus is full, the butcher nature is rich, assumes the pink, the nucleus is a purple red, comes, the nuclear shape varies, and may present the binuclear or the polynuclear phenomenon, the nucleolus is clear, the cell growth is exuberant, has not seen perishes weakly the minute.Experiment group:after e the technique oil affects 48hours, cells'form change obviously, cells which paste the wall reduce, cells swell, the volume increases, edge coarse anomalous, the butcher nature concentration, in the cytoblastema presents the dense granule, the integrity of cell membrane is detroyed, a part of cells presents the karyopyknosis, has not seen obviously perishes weakly the minute.3.Immunity group reduction exam the cell Bcl-2 and Bax two kinds of protein expression situation, compare with the control group, the expression of Bax protein in experiment group increase obviously, the diffirence has Statistics significance, but the expression of the expression of Bcl-2 protein in experiment group decrease obviously.Conclusion1. e the technique oil has the function which to person ovary cancer cell SKOV3 the obvious multiplication suppresses and induces perishes weakly.2.in the process of e the technique oil induces the person ovarian cancer SKOV3 cell to perish weakly, it probably has something to do with decline Bcl-2 the expression as well as upward Bax the expression.