Effects Of Oral Carcinoma-associated Fibroblasts On The Biological Behavior Of A Vascular Endothelial Cell Line

[Background] Carcinoma-associated fibroblasts (CAFs),which present distinctmorphological, biological characteristics and function,are regarded as the primary.host mesenchymal cells in tumor-host interface microenvironment. Vascularendothelial cells (VEC) are the target cells of angiogenesis that has direct relationwith tumor angiogenesis. While tumor angiogenesis is regulated by the rigorous andcomplicated signal network in the microenvironment, numerous previous researchfocused on tumor cells. Until recently, CAFs have been verified playing animportant role in recruitment, proliferation of VEC and vascular bud shaping in thestudies of breast and prostate carcinoma. However, no reports about the effects ofCAFs on angiogenesis of OSCC has been published. Our previous work hassuccessfully obtained purified oral CAFs and focused on its effect on the lingualcarcinoma cell line and matrigel. Based on these results, our current study wasaimed to preliminarly explore the effect of oral CAFs on the biological behavior ofVEC.[Purpose] By establishing interaction models between CAFs and a human vascularendothelial cells line(ECV304):(1) to observe the effects of oral CAFs on themorphous and proliferation of ECV304;(2) to observe the effects of oral CAFs onthe random migration and chemotactic migration of ECV304.[Methods] (1) The primary oral CAFs and normal fibrolasts (NFs) of oral mucosawere obtained by tissue culture.; the effects of oral CAFs on the morphous and viability of ECV304 were observed through morphological observation and MTTassay by establishing interaction models between oral CAFs and ECV304 (2) Theeffects of oral CAFs on the random cell movement of ECV304 were observedthrough half-cells erasion assay, and CAFs and ECV304 were co-cultured in theTranswell cell culture insert system to detect the chemotactic migration of ECV304.[Results] Oral CAFs and NFs were separated, cultivated, purified and identifiedsuccessfully. ECV304 that co-culture with CAFs presented obviously morphologicalalteration. Compared with NFs, oral CAFs remarkably enhanced the viability ofECV304 at both 24h and 48h(P＜0.05), oral CAFs remarkably elevate randommigration of ECV304 at 24h(P＜0.05) and oral CAFs remarkably elevatechemotactic migration of ECV304 at both 12h and 24h(P＜0.05).[Conclusions] Oral CAFs could promote the proliferation and migration of a humanvascular endothelial cells line (ECV304) in vitro. For the first time, this study hasexplored the effects of oral CAFs on the biological characteristics of vascularendothelial cells. The results are of great benefits to understand the effects of CAFson angiogenesis, and provide a valuable evidence for further study on themechanisims of the effects of oral CAFs in angiogenesis.