Experimental Study On Effect Of PNS For Venous Congestion And Repair Of Tissue Of Rabbit Skin Flap Transplantation

Objective:To study the impact of venous congestion and tissue repair of PNS act on rabbit flap surgery,and observe its effect on serum VEGF, b FGF expression.Method: Healthy clean adult New Zealand rabbits were 32, male or female, not pregnant female rabbits, weighing 2.0 ~ 2.5Kg, provided by the Lanzhou Institute of Biological Products Laboratory Animal Room, License number: SCXK(Gan) 2012- 0001. The 32 rabbits were randomly numbered grouped into four groups: A group, namely the experimental group(after intragastric administration of 200% of total saponins of Panax solution 50 mg /Kg intravenously, 1 times / day for 5 days); group B, which positive control group(after the model given dextran 40 injection 18 ml / Kg intravenously, 1 times / day for 5 days); group C,namely the control group(made congestion model and normal saline 18 ml / Kg intravenous injection, 1 times / day for 5 days). These three groups can be made per rabbits abdominal flap congestion model 2, a total of 48 flaps model, in which the flap 24 general observation,drawing flap 24; D group, sham operation group(made of rabbit abdominal surgery incision,not flap sutured; no medication) only serum factor before and after surgery. Above each group before the first seven days of modeling and heart blood, after the model after model given regular medication, continue feeding, observation flap healing, PHOTOSHOP software metering flap necrosis area, respectively, after the first one hour The first 24 hours, 7 days a total of three-phase, cut the flap near, middle, distal region of 0.5cm × 0.5cm skin tissue blocks as a specimen, HE staining light microscopy histological changes and detection of tissue superoxide dismutase(SOD) activity, myeloperoxidase(MPO) content by ELISA serum VEGF, b FGF expression.Results: 1, The seventh day after surgery, A group of skin flap necrosis area was 9.37 ±1.63(cm2), group C flap necrosis area was 14.49 ± 3.56(cm2), the two groups were statistically significant(P<0.01). 2, The seventh day after surgery, the flap HE staining,microscopic pathology was observed, showing A, group B segments flap tissue angiogenesis,fibroblast proliferation obvious, skin edema and inflammatory granulocytes diffuse to a lesser extent in group C. 3, After 1h, 24 h, 7d different time taking tissue flap taxidermy, compare SOD, MPO detected values, the results show: comparison within groups, between each flapnear the distal, SOD、MPO activity and content, the difference was statistically significant(P<0.01). Between the three groups, group A and B flap near, medium, distal SOD values??were higher than the group C, with a statistically significant(P<0.01); the,group A and B flap near, middle and distal MPO activity was lower than in group C, with a statistically significant(P <0.01); comparison between group A and group B was not statistically significant(P> 0.05). 4, Each group postoperative serum VEGF 、b FGF were higher than preoperative values; the postoperative serum VEGF, b FGF of group A and B were higher than group C and D, with statistically significant(P<0.01). Comparison between group C and group D was not statistically significant(P> 0.05).Conclusion: PNS by increasing SOD, MPO, to significantly improve local skin metabolism, oxygen free radical clearance effects; PNS can induce the expression of VEGF,flap formed in early postoperative promoting collateral circulation, capillary and increased blood flow to the improve the venous blood stasis; PNS can enhance the b FGF expression and promote fibroblast proliferation, enhance tissue repair ability of trauma; PNS can increase the distal end of the flap blood supply and promote flap survival and for skin flap transplantation enlarged has an important significance in clinical application.