The Expression Of Survivin And PTEN In Cervical Cancer And Associated Research

Objective Cervical cancer is a common malignancy among women, ranked second among in the world of women malignant tumors. Survivin, seems to be involved in the critical steps of cancer onset and progression and may be associated with unfavorable clinical outcome.Loss of PTEN expression has been associated with tumor progreeion and adverse patient outcom. The goal of this study was to analyze the expression levels of Survivin and PTEN in normal cervical tissue, cervical intraepithelial neoplasia (CIN) and cervical carcinoma, and to analyze its relationship with clinicopathological parameters of cervical cancer-related links to explore the above-mentioned target happened in cervical cancer development, invasion and metastasis, for the diagnosis and treatment of cervical cancer to provide a new and effective target.Methods Using streptavidin-biotin-protein-linked peroxidase method (ie, SP) immunohistochemical method to detect the expression of Survivin and PTEN in 15 cases of normal cervical tissue,37 cases of CIN tissue (12cases of CIN I tissue,12 cases of CINⅡtissue,13 cases of CINⅢtissue),48 cases of cervical cancer. Analysis of cervical cancer in Survivin and PTEN expression and clinical stage, histological grade and other clinicopathological parameters of relevance, at the same time the relationship between Survivin and PTEN was studied in the development of cervical cancer for a preliminary study.Results 1. Survivin were expressed mainly in cancerous lesions, but also expressed in some normal tissues.At the same time, PTEN were expressed mainly in some normal tissues, but also expressed in cancerous lesions.2.Positive expression rate of Survivin in normal cervical tissues, CIN, cervical cancer tissues were 6.67%,54.05%,83.33%,the trend has gradually increased (P<0.05). Among them, significant difference was existed between normal cervical group and CIN group(P<0.05), also between normal cervical group and cancer group(P<0.05), CIN group and cancer group(P<0.05).3.Positive expression rate of PTEN in normal, cervical tissues, CIN, cervical cancer tissues were93.33%,51.35%,29.17%, the trend has gradually increased (P<0.05). Among them, significant difference was existed between normal cervical group and CIN group(P<0.05), also between normal cervical group and cancer group(P<0.05), CIN group and cancer group(P<0.05).4. In cervical carcinoma, positive rate of Survivin protein expression at FIGO stagesⅡb～Ⅳperiod was higher than theⅠ～Ⅱa period (x2=8.817, P<0.05), with lymph node metastasis group was higher than that without lymph node metastasis group (x2=4.000, P<0.05); Expression of Survivin in cervical cancer were not related with age, the degree of differentiation, histologicaltypes (P> 0.05).5. In cervical cancer, positive rate of PTEN protein's expression at FIGO stages II b～Ⅳperiod was higher than theⅠ～Ⅱa period (x2=5.557, P<0.05), with lymph node metastasis group was higher than that without lymph node metastasis group (x2=13.341, P<0.05).Expression of PTEN in cervical cancer were not related with age, the degree of differentiation(P> 0.05).6. The relationship between Survivin and PTEN was positively correlated, and the correlation coefficient rs=-0.753(P<0.05).Conclusion Survivin in the normal cervix, CIN, cervical carcinoma was higher trend,but the PTEN has a declining trend; Survivin in the phaseⅡb～Ⅳ, there is lymph node metastasis of cases of cervical cancer is higher than theⅠ～Ⅱa period, no lymph node metastasis; PTEN in the phaseⅡb～Ⅳof cervical cancer is higher than theⅠ～Ⅱa period; There is synergy between Survivin and PTEN; Survivin and PTEN plays an important role in cervical cancer invasion and metastasis, Combined detection of Survivin and PTEN may be a new target of cancer diagnosis and treatment and a new way to assess the severity of cervical cancer.