| Aconitum carmichaelii is a kind of Traditional Chinese Medicine with hadro-cardia, boosting voltage, analgesia and anti-inflammatory. It has being used in clinical widely. The toxicity element of the aconitum is aconitine , which with the toxicity of the central nervous system and cardiovascular system usually. There is no report in respiratory apparatus tocicity. In order to understand the influence of aconitum to the respiratory system , the research was conducted in vivo and in vitro .Experiment in vivo include the SD rat repeated toxicity study and Beagle dog safety pharmacology study. The SD rat was administrated daily for 3 month followed by a one month recovery phase. The study included a negative control group and 9 dosing groups(Raw Common Monkshood Mother Root 13.0, 6.5 and 3.3 g raw drug /kg; Raw Kusnezoff Monkshood Root 8.3, 4.2 and 2.1 g raw drug /kg; Salted aconite root 6.18, 3.09 and 1.55 g raw drug /kg). There are 30 rats in every group with half male and hafe female. The result showed that the difference of the lung tissue'organ coefficient has statistical significance (P<0.05) compared with the control group in the high dose group of the raw Kusnezoff Monkshood Root, the other groups have no significance different. The histopathology examination of the lung displays that no apparent abnomal in all rats.The Beagle dog safety pharmacology study was conducted in one dosing group.There are 3 male and 3 female dogs in the group.Raw kusnezoff monkshood root (2.94 g raw drug/kg) was administrated after the dogs in anaesthesia. The breathing frequency and breathing depth were tested at before dosing and 30, 60, 90, 120, 180, 240min after dosing. The result showed that the breathing frequency and breathing depth are significant higher compare to before dosing (P<0.05) at 120min,180min,240min after dosing in male dog. No any abnomale change in female dogs.The fetus lung fibroblast cell and CHL cell were used in the vitro study. The fetus lung fibroblast cell was got from 19 day old fetus rat. Culturing the cell and aconitine( 50,25,5,2.5,0.5ng/ml) together for72h. The cell oxidative damage and promote apoptotic effect were tested. The result showed: No cell oxidative damage and promote apoptotic effect in all aconitine groups.Chinese hamster lung fibroblast cell (CHL cell)test: CHL cell was used to determined the DNA damage by the aconitine(100,50,25,5μg/ml) for 2h culturing . the result displayed that the difference of the trailing cell rate and the trailing cell length have statistical significance (P < 0.05) compare to the negative control in the metabolism activation system and non-metabolism activation system when aconitine with 100,50μg/ml. There is dose response relation between the aconitine and the trailing cell rate and the trailing cell length.Integrated the vivo and vivtro test , we can make a conclusion as following: The lung tissue of SD rat don't been damaged by the raw Common Monkshood Mother Root (13.0 , 6.5, 3.3g raw drug /kg) , raw Kusnezoff Monkshood Root ( 8.3, 4.2, 2.1g raw drug /kg )and raw salted aconite root (6.18, 3.09,1.55g raw drug /kg). The crude kusnezoff monkshood root (2.94 g raw drug/kg) make the male beagle dogs breathing frequency speeds up and breathing depth augment. The aconitine(50,25,5,2.5,0.5ng/ml) don't make the oxidative damage and the promote apoptotic. The CHL cell DNA damage caused by aconitine with 100,50μg/ml. |
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