Effect Of Transurethral Resection Of The Prostate(TURP) And High Intensity Focused Ultrasound(HIFU) On The Expression Of Caspase-3,Caspase-7 And α₁A-ARmRNA In Prostate Tissue

Objective: Observe Transurethral Electrovaporization of the Prostate(TUVP) and High Intensity Focused Ultrasound(HIFU) on the change of caspase-3,caspase-7 andα1A-ARmRNA in prostate tissue,discuss the effect of thermal damage on the expression of apoptosis effective factor caspase-3,caspase-7 andα1A-ARmRNA in prostate tissue.Methods: In our study we enrolled 30 patients with symptomatic benign prostatic hyperplasia (BPH) undergoing TUVP and 6 BPH patients were treated by HIFU as experimental group;we also enrolled 15 BPH patients undergoing suprapubic prostatectomy as control group. Immunohistochemistry methods were used to detect the expression of caspase-3,caspase-7, according to expressive positive rate, staining were grated on:"-"<10%,"+"10%～30%,"++"31%～70%,"+++"> 71%. Though RT—PCR method detect the expression ofα1A-ARmRNA in sample.Results : 1.Make use of immunohistochemical method detect expression of caspase-3,caspase-7 in every group sample.Find the expression of caspase-3,caspase-7 in control group is obviously fewer than TUVP group(P<0.01),HIFU group between in the two groups.They almost express in prostatic secretory epithelium and stroma cell,but seldom seen in prostatic smooth muscle cell.2. Make use of gel Image Processing system analysisα1A-AR/β-Actin/β-action have difference in every group.The relative value of control group is maxium,TUVP group is minimum,HIFU group is between in the two groups.(P<0.05) Conclusion :Our study supports TUVP and HIFU etc. thermal damage treatment can promote prostate tissue apoptosis.