The Protective Function Of ω-3PUFA On The Intestinal Barrier Of Rats With Obstructive Jaundice

The obstruction jaundice is a common pathology process of many kinds of disease in liver and gall surgery. The intestinal bacterium have lost the inhibitory function of choler and they have grow overly. It makes the bacteria colony disorder. The disorder bacteria colony may damage the gastro -intestinal mucus cell and may make the intestinal mucous membrane permeability to increase, which causes intestinal bacterium shifting. Simultaneously, the over growth bacteria colony produces the massive metabolite and the toxin, which have lost the choler acid adsorption degeneration function and the intestinal barrier function are absorbed massively into the blood. It forms the endotoxemia. After the massive endotoxins enter the blood circulation, the endotoxemia destroys the intestinal mucous membrane barrier function and promotes bacterium shifting. Finally, the vicious circle will certainly cause the multi-internal organs damage. Nowadays, reduce the endotoxemia, weaken the endotoxin biology activeness and protect intestinal barrier function has become a main research direction to reduce the obstruction of jaundice complication.When the intestinal mucous membrane receives the stimulation the mucous membrane then produces SIgA which partially, suppresses the intestinal bacterium adherency intestinal mucous membrane surface, especially to G^-. SIgA avoids the bacterium penetration intestines epidermis having the transposition. Simultaneously can neutralize biological activity antigen, virus and so on. Toxin and enzyme are the intestinal immunity barrier function important constituent.ω-3 multi-unsaturated fatty acid (ω-3PUFA ) is a kind of main origin in the marine life important nutrient, which has close correlations with organism fats metabolism. In recent yearsω-3 multi-unsaturated fatty acid(ω-3PUFA ) has received scientists' universal attention to the human body physiological action day by day. Not only the research discovers EPA and DHA can maintain the factor which the cerebrum and the nervous system must, falls the blood fats, the anti-thrombus, preventing and controlling cardiovascular disease, but also has the adjustment internal secretion, sharpens the immunity ability. It is becoming the important constituent of the nutrition immunity preparation.Purpose:Search the effect and potential mechanism ofω-3PUFA on the intestinal barrier of rats with obstructive jaundice.Methods: Take 40 grown-up health rats, feed a week and adapt environment. 40 rats is divided into three groups stochastically. Stipulate A group is the sham-operation group, B group is tied up choledoch and feed normally, C group is tied up choledoch and feedω-3PUFA additionally. A group and B group are feed Standard fodder and free potable water five days. C group is feed Standard fodder and filledω-3PUFA 100 mg daily in front of technique, total five days. The maintenance experimental group condition is consistent and prevents the group difference. B and C groups are tied up choledoch and duplicated obstruction jaundice model. A group is separated choledoch but not gaven tie up. C group is filled continulyω-3PUFA 100 mg daily from technique late to execution.B and C groups are killed selected material after the technique seventh days,14th days separately. A group is killed selected material after the technique seventh days. Examination target: 1,Venous blood,the liver, the spleen organize,the mesentery lymph node organizes bacilliculture, microscopic exam and calculate each group of big mouse bacterium shifting rate. 2,LAL quantitative determine blood plasma endotoxin. 3,Observation intestinal mucous membrane shape. 4,SABC determine small intestinal mucous membrane inherent lgA⁺ Cell quantity.Results: B and C groups' urine starts to change yellow , dejecta changes shallow, orexis reduces, activity reduces, sleep increases. Tail acme and ear acme changes yellow about seven days. A group restores normal basically, orexis improves after technique second day. B and C groups ligation spot above choledoch saccate expands, the choledoch wall changes thin after technique 7th,14th day.B group compares the endotoxin content increase with A group to be obvious, the difference has the significance; C group compares the endotoxin content increase with A group to be obvious too, the difference has the significance; C group compares the endotoxin content level with B group to be low, the difference has the significance.A group has not had bacterium shifting, B and C groups have had bacterium shifting.C group compares bacterium shifting rate with B group to reduce obviously. B_14d group and B_7d group compare the bacterium shifting rate increase. C_14d group and C_7d group compare bacterium shifting rate also to have the increase.Observes under the light microscope, A group's ileum down structure integrity, the length is even, intestines wall myo- level thickness is even, serosa surface integrity. B group intestinal mucous membrane changes obviously thin, serious only approximately is A group intestinal mucous membrane thickness 1/3. The intestinal villus sparse, thinning, atrophy, break, the down drops highly, the structure is non-uniform, the superficial absorption cell and the cup-shaped cell reduce, some obviously inflammatory cell infiltration. C group intestinal mucous membrane changes the thin change B group to have improves obviously, the intestinal villus sparse situation also is improved.B group compares the intestinal mucous membrane inherent level lgA⁺ cell quantity reduction with A group to be obvious, the difference has the unusual significance; C group compares the intestinal mucous membrane inherent level IgA⁺ cell quantity with A group also to reduce, the difference has the significance; C group compares the intestinal mucous membrane inherent level lgA⁺ cell quantity with B group to increase, the difference has the significance.Conclusion: 1,Under the obstruction jaundice condition the intestinal mechanical barrier function damage, the blood plasma endotoxin level and the bacterium shifting rate markup, in the intestinal mucous membrane inherent level lgA⁺ cell reduces, and with blood plasma endotoxin level and bacterium shifting rate inverse correlation.2,ω-3 unsaturated fatty acid (ω-3PUFA ) may reduce the intestinal mechanical barrier function harm which causes by the obstruction jaundice, increases the intestinal mucous membrane inherent level lgA⁺ cell quantity, reduces the intestinal bacterium shifting rate, has the improvement function to the obstruction jaundice prognosis.Analysis: The reasons cause obstruction jaundice organism endotoxemia and the bacterium shifting possibly includes: Intestinal bacterium factor. Organism immunity factor. Intestinal barrier factor. The intestinal barrier mainly includes: (1) Mechanical barrier. The mechanical barrier is made by the intestinal tract mucous membrane epithelial cell, the intercellular space close connection and the membrane three constitutions, which can prevent the bacterium penetration mucous membrane to enter the depth portion organization effectively. (2) Biological barrier. The intestinal tract resident bacteria colony is the micro ecosystem which it is an interdependence affects mutually. This kind of micro ecological equilibrium consist of the intestines biology barrier. (3) Chemistry barrier. Chemistry barrier is made by secretes into the gastro-intestinal tract chemical substances, gastric juice, bile, bacteriolysin, mucopolysaccharide and albuminolysis enzyme and so on, which is composed, has chemistry sterilization. (4) Immunity barrier. The immunity barrier is constituted by the intestines correlation lymphatic tissue, including T lymphocyte, B lymphocyte and cytoplasmic cell. The immunity function is produced by IgA, IgG, IgM, in which mainly is IgA. IgA is existence in the intestinal tract SigA. Its function is neutralization biological activity antigen, which include virus, toxin,enzyme original and so on. In the intestinal juices SIgA comes from the bile and in the intestinal mucous membrane inherent level lgA⁺ cell, which is specially under the obstruction jaundice condition the intestinal barrier damage, may have the endotoxemia and bacterium shifting.The intestinal bacterium and the endotoxin enter the blood to be able to aggravate the intestinal barrier damage, forms the vicious circle.This experimental result shows that under the use of obstruction jaundice conditionω-3 multi-unsaturated fatty acid (ω-3PUFA )to be possible to improve the mucous membrane to change thin and the intestinal villus sparse situation effectively. It can reduce endotoxemia and reduce bacterium shifting rate. The experimental result analyzes applicationω-3 multi-unsaturated fatty acid (ω-3PUFA ) to improve mucous membrane damage situation, reduce the endotoxemia, reduce the bacterium shifting rate possible mechanism for its immunity adjustment and the immunity enhancement function. The experimental result shows that B and C groups of small intestinal mucous membrane inherent level lgA⁺ cell quantity compares with A group reduces, simultaneously blood plasma endotoxin level and bacterium shifting rate increases. C group of intestinal mucous membrane inherent level IgA⁺ cell quantity compares with B group increases, the blood plasma endotoxin level and bacterium shifting rate compares with B group reduces. From this infer thatω-3 multi-unsaturated fatty acid (ω-3PUFA) may strengthen the intestinal immunity barrier function, thus may reduce the blood plasma endotoxin level and bacterium shifting rate. Because it is reduced the bacterium endotoxin level, the endotoxin reduced to the intestinal mucous membrane harm function. Thus it enables the intestinal mucous membrane shape structure the harm to improve, then indirect protects intestinal mechanical barrier function. Moreover,ω-3 multi-unsaturated fatty acid (ω-3PUFA) strengthens organism immunity defense function, strengthens the liver reticulo-endothelial system and the neutral granular cell function. Possibly, it is one of reasons which the blood plasma endotoxin level and bacterium shifting rate reduces.