Preparation Technology Of Section Anatomy Atlas And Quality Improving Of Artery Boundary For Digital Rat

The study about digital rat has great significance impact on the improvement of the research about both laboratory animal and life science, and it's primary task is to construct section anatomy atlas with high quality. So this thesis introduced the primary research about preparation technology of section anatomy atlas for digital rat.Firstly, we introduced the preparation technology of section anatomy atlas for digital rat from the following aspects: sample selection, pretreatment, cryofixation, embedding, cutting, and raw image acquisition. By this preparation technology we constructed a section anatomy atlas for digital rat. Comparing with some other kinds of section anatomy images, we analyzed the advantages and disadvantages of the atlas. Contrasting to the images which acquired in earlier experiments, we indicated some key factors to construct high quality atlas, and putted forward some improving techniques of preparation technology.Then we finished segmentation of outline by photoshop software. The 3D model of outline was constructed by visualization program which developed based on VTK This model can reflect the structure and shape of rat accurately, and it confirmed that the preparation technology can acquire high quality section anatomy atlas.Finally, we did some improvements in the sample preparation in order to improve quality of artery boundary for digital rat. The special injection medium was perfused into arterial blood vessels of SD rat, evaluating the quality of perfusion by the digital radiography system at the same time. A digital rat atlas of sectional anatomy with artery vessels perfused was achieved lastly, and the 3D model of arterial system was built by the segmentation and visualization programs. Microstructure of arterial system of digital rat can be obtained accurately from this 3D model.