Study On Relation Between Polymorphism Sites Of Tim-3 And Rheumatoid Arthritis

Object To investigate The relation between two single nucleotide polymorphism sites of T cells immunoglobulin domain and mucin domain protein-3(Tim-3) and rheumatoid arthritis(RA) in adult population of the Han nationality from Hubei Province of China.Methods The polymorphism were detected with polymerase chain reaction–restriction fragment length polymorphism (PCR-RFLP) and allelic specific polymerase chain reaction(ASPCR). The genotype and alleles frequencies were calculated and analyzed. Rheumatoid factor(RF)was detected with immunoturbidimetry and C reactive protein(CRP)and Cyclic citrullinated peptid(CCP) antibody were detected with ELISA.Results 1. The genotype frequencies of C/C,C/T and T/T in–1541 C>T polymorphism were 0.960,0.040 and 0 respectively in healthy population and were 0.948,0.052 and 0 respectively in RA population. No significant difference in genotype and alleles frequencies was found between RA patients and control subjects (P>0.05).2. The genotype frequencies of T/T,T/G and G/G in 4259 T>G polymorphism were 0.960,0.040 and 0 respectively in healthy population and were 0.870,0.130 and 0 respectively in RA population. There are significant differences in genotype and alleles frequencies between RA patients and control subjects (P=0.020,P=0.023).3. There are extreme significant differences in genotype and alleles frequencies between RA patients of the Han nationality from Hubei Province of China and those of Korea (P<0.001 respectively).4. No significant difference in levels of RF,CRP and CCP antibody respectively in different genetype of RA patients (P>0.05).Conclusion There are polymorphism sites in the promoter region and the third exon of Tim-3,and the latter one is associated with rheumatoid arthritis in adult population of the Han nationality from Hubei Province of China, but this polymorphism does not influence the level of RF,CRP and CCP.