The HPLC Fingerprints Analysis Of Coptis Teetoides And C. Teeta

Coptis Chinensis Franch. from Coptis genus of Ranunculaceae family, is one of the most important Chinese Traditional Medicines. The officinal part is its rhizome. According to the literature and investigation , there are about sixteen species of Coptis genus in the world. In China, cultivated species and wild species such as C.deltoidea C.Y. Cheng et Hsiao, C. omeiensis (Chen) C.Y. Cheng, C.teeta Wall and C.teetoides C.Y. Cheng have been used for treatment. Referring to the classification and Latin names of C.teeta Wall. and C. teetoides C.Y. Cheng ,different scholars have different viewpoints.Original plants and medicinal materials are studied in morphological features. The foliages and rhizomes are investigated in inner structures and microscopic characters.The comparison with TLC appears that palmatine could be considered the characteristic component for their TLC identification.According to Chinese Traditional Medicine Injection Fingerprints Chromatogram Technique Demand, employing RP-HPLC as a method, 20 samples, from different producing areas of YunNan and Tibet, have been analyzed. With the reference of Coptisine peak, 11 peaks have been identified as common ones. The HPLC-FPS of C. teeta Wall and C. teetoides C.Y. Cheng have been established. The result suggests that such an HPLC-FPS can well reflect the characteristics of C. teeta Wall and C. teetoides C.Y. Cheng.and can be used as a scientific means to identify the original plants of C. teeta Wall and C. teetoides C.Y. Cheng.In addition,20 samples from different producing areas have been analyzed by means of RP-HPLC, The contents of berberine and four other alkaloids have been obtained. The result shows that the contents of Palmatine in C. teetoides C.Y. Cheng are obviously higher than those in C. teeta Wall ,and the content of epiberberine in C. teeta Wall and C. teetoides C.Y. Cheng are too low to be analyzed.