Multi-components Quantitation By Single Marker For Quality Evaluation Of Coptis Chinensis And Panax Ginseng

To evaluate comprehensively the quality of herbal medicines is a challenge because of their diversity of chemical ingredients. The quality-control approach for simultaneous determination of multiple components had been developed in recent years and became to one of the main quality-control methods. However, current quality control pattern are limited to industrial application, for most of the natural chemical reference substances are unavailable commercially, for it requires sufficient chemical purity standards or chemical reference substances. In addition, high purity chemical standards or chemical reference substances of herbal medicines are expensive and insufficient, especially when the component was of low content level and hard to be purified from the plant. It was urgently necessary to develop a convenient and low-cost approach for controlling the quality of herbal medicines. Herein, we proposed a method, quantitative analysis of multi-components by single marker (QAMS), in herbal medicines. Among them, one component was determined with external standard method, while the other components were calculated by their relative correction factors (fs/k) at specific wavelength. Then the result of the proposed method was valid by comparing the contents of the components which were authentically determined from external standard method. In this paper, two herb medicines were used to valid this method and it was proved to be a fast, convenient, and accurate method.Five main alkaloids, berberine, jatrorrhizine, epiberberine, coptisine and palmatine, were selected as markers to evaluate the quality of Coptis chinensis. And Within the linear ranges, the values of fs/k of berberine to jatrorrhizine, epiberberine, coptisine, palmatine were 1.11,1.03,0.966 and 1.03, respectively. The fs/k had a good reproducibility in various instruments, chromatographic columns (RSD=1.4-4.4%). The average recovery rate and RSD(%) of jatrorrhizine, epiberberine, coptisine, palmatine and berberine were 101.0(2.4).100.6(1.6).99.5(1.8).101.6(2.5) and 103.7(1.3), respectively. The contents in 28 batches of samples, collected from different areas and species, were determined by both external standard method and QAMS. No significant differences were found between two methods. It is feasible and suitable method to evaluate the quality of rhizome of Coptis chinensis.Using ginsenoside Rb1 as the contrast, the fs/k of the other eight ginsenosides were determined by HPLC-DAD. Within the linear ranges, the values of RCF of ginsenoside Rb1 to ginsenoside Rg1, Re, Rf, Rh1, Rc, Rb2, Rb3 and Rd were 1.40,1.23,1.49,1.82,0.937,1.01,1.18 and 1.15, respectively. The RCF had a good reproducibility in various instruments, chromatographic columns (RSD=0.4-3.9%). The average recovery rate and RSD(%) of Rg1, Re, Rb1 Rf, Rc, Rb2, Rb3 and Rd were 103.5(2.4). 101.5(5.0).101.9(4.3).99.2(4.2).97.5(2.7).101.1(4.8).105.2(7.6)å'Œ103.0(2.2), respectively. According to their fs/k, nine ginsenosides (ginsenoside Rg1, Re, Rf, Rh1, Rb1, Rc, Rb2, Rb3, Rd) in P. ginseng and four ginsenosides (ginsenoside Rg1, Rh1, Rb1 Rd) in P. notoginseng were simultaneously determined by QAMS. The results of QAMS method were validated by comparing with that of external standard method, and no obvious significance was found.