Time-resolved fluoroimmunoassay (TRFIA) is an ultrasenstive analytical method, instead of radioimmunoassay, it has been widely used in diagnostics and clinical analyses, iatrology, life sciences.There exist two directions: DELFIA and DSLFIA in the field of TRFIA research. DELFIA is named by using enhanced-solution when measuring. High sensitivity can be obtained with DELFIA, but the operation is inconvenient. DSLFIA is widely concerned because of operating conveniently and the appling foreground in DNA hybridization assays and immunoassay fluorescence image.In this dissertation, there was a big paragraph discussed the synthesis of bipyridinery intermediates Tetraethyl 2,2′,2′′,2′′′-[(4,4′-Dibromo-2,2′-bipyridine-6,6′-diylbis(methyleneni- trilo)]tetrakis(acetate),which applied to solid-phase time-resolved fluoroimmunoassay. The condition of the synthesis was researched. We studied the reacted temperature, reacted time, catalyst amount, quomodo of adding material, propirtion of material, and improved the method of synthesis, and enhanced the yield.
|