| Murraya panaculata (L.) Jack , a well-known medicinal plant, is widely used for treatment of stomachic diseases, inflammation, different body pains, trauma , bleeding caused by internal and external injury etc. It was been also used as a tonic and haemostatic agent. Due to the demand for its important medicinal use in China, it was largely cultivated in Yunnan and Guangxi Provinces.The alkaloids, terpenoids and coumarins in this plant have been reported more before, but the flavonoids reported fewer. For the sake of the better use of this herb medicine, this paper describes the extraction, isolation, determinations and the measurement of the flavonoids in the dried leaves of Murraya panaculata (L.) Jack.Extraction and SeparationDried leaves (3kg) were extracted by heat reflux in 85% eth-anol (15L, 12L,and 10L respectively) for three times (2h, 1.5h and1h, respectively). The combined extract was evaporated to dryness under reduced pressure to yield a greenish viscous syrup. 100g ext-racts had been weighed and dissolved in water and absorbed by macroporous resin, then eluted with 95%EtOH. The extraction was subjected to silica gel column chromatography using ethyl acetate-petroleum ether as mobile phase to afford compounds A(11.2mg),B(12.6mg),C(11.3mg),D(10.4mg),E(11mg),F(6.4mg),G(2.9g)and H(1.3g)。All the compounds was identified respectively by 1DNMR and 2DNMR as 5, 6, 7, 3′, 4′-pentamethoxyflavone(A); 5, 7, 8, 3′, 4′- pentamethoxyflavanone(B);5-hydroxy-6,7,8,3′,4′pentamethoxyflavone(C);3′,5′-dihydroxy-7,4′-dimethoxyflavone(D);7-hydroxy-3′,4′,5-tri-methoxyflavone(E);5,7,3′,4′-tetramethoxyflavone(G);5,7,3′,4′,5′-pentamethoxyflavone(H). Compound F is not identified now and hoped to be identified in the future. But for E,F ,G and H , all the compounds were isolated the first time from Murraya exotica L..Measurement of flavonoids in the taditional chinese medicine of Sanjiuweitai.In this paper we present a more sensitive quantification method by HPLC for analyzing the main flavonoids (G and H) in the traditional chinese medicine of Sanjiuweitai.HPLC was performed on a ZORBAX C18 column(250 mm×4.6 mm,5μm), using 35% acetonitrile and 65% water as mobile phase at 25℃with the flow rate of 1.2 ml/min.ConclusionsⅰThe linear range of 5,7,3′,4′-tetramethoxyflavone is 0.4μg～20μg. y=2885.97x +73.97 r=0.9999The linear range of 5,7,3′,4′,5′-pentamethoxyflavone is 0.4μg～20μg. y=2072.24x-65.12 r=0.9999ⅱ5,7,3′,4′-tetramethoxyflavone solution was injected into HPLC for six times, the RSD of precision tests is 1.36%(n=6).5,7,3′,4′,5′-pentamethoxyflavone solution was injected into HPLC for six times, the RSD of precision tests is 1.38%(n=6).ⅲThe repeatability tests for six samples showed RSD are 0.99%(com pound G, n=6) and 1.17%(compound H, n=6) respectively. ⅳWhen the concentration of 5,7,3′,4′-tetramethoxyflavone, 5,7,3′, 4′,5′- pentamethoxyflavone solution descrease to 0.0002mg/ml, S/N=3.2 after determined by HPLC, So the detectability is 0.004μg。ⅴThe test of stability showed RSD are 0.70% and 1.74% respectly,so the 5,7,3′,4′-tetra methoxyflavone, 5,7,3′,4′,5′-pentamethoxyflavo- ne solution is stable in twelve hours .ⅵThe average recovery are 100.6%(compound G) and 102.6%(comp- ound H) respectively.The content of the two flavonoids-compounds G and H in the Sanjiuweitai. getting from different batches were determined by the above method, the results showed that their contents were stable in different batches. The content of G and H in Sanjiuweitai were 24.25ug/g and 19.79ug/g.The results of this paper can offer new items of content determination for quality standard establishment of Murraya panaculata (L.) Jack. and its agents。Also, the results can offer science foundations for the development and utilization from this medicine plant. |
PDF Full Text Request