Expression Of TGF-β And Its Signal Proteins In Epithelial Ovarian Tumour

Epithelial ovarian cancer is well known for its highest death rate in mutton tumors. So it has been paid more attention to investigate the mechanism of epithelial ovarian tumor day by day. The TGF-βsignaling pathway will interrupt if one of its factor inactive, which will induce emergence, development and metastasis of tumor. The roles of TβR-Ⅱand Smad4 are the most important in this pathway .So that we collect clinical samples in several hospitals to observe the expression of TGF-β, TβR-Ⅱand Smad4, to investigate the relationship among the three factors, and to discuss the probable action mechanism in the emergence and development of epithelial ovarian tumor.1. Materials and MethodsTissues of epithelial ovarian tumor were obtained from 102 patients who underwent surgery (wax). The age of patients ranged from 28 to 69 years old (mean: 50years old). Serous (n=45), mucinous (n=29), endometrioid (n=18), clear cell carcinoma (n=10); GradeⅠ/Ⅱ(n=56), GradeⅢ/Ⅳ(n=46);Lymph node metasasis (n=42), non-lymph node metastasis (n=60) ; ascites (n=49), non-ascites (n=53). 30 cases of normal ovarian tissues were used as control. Use the immunohistochemical staining (SP methods) to detect the expression of TGF-β,TβR-Ⅱand Smad4. Choose 5 high powerfields (×400), observe 100 cells per powerfield,take count of positive staining cells of 500 cells under microscope to estimate the expression situation of TGF-β,TβR-Ⅱand Smad4 in epithelial ovarian tumors and in normal ovarian tissues. Compare the expression differnces of the three factors in epithelial ovarian tumors and in normal ovarian tissues.Staistical analysis was executed by SPSS 13.0 Software, x2 test was adopted. P<0.05 were considered significant.2. Result:1) The positive expression rates of TGF-β,TβR-Ⅱand Smad4 in normal ovarian tissues are respectively 46.7% (14/30), 66.7% (20/30) and 63.3% (19/30).2) The positive expression rates of TGF-β,TβR-Ⅱand Smad4 in epithelial ovarian tumors are respectively 67.6% (69/102), 22.5% (23/102) and 37.3% (38/102).3) The positive expression rates of TGF-βin epithelial ovarian tumors are higher than those in normal ovarian tissues (P<0.05); the positive expression rates of TβR-Ⅱand Smad4 in epithelial ovarian tumors are lower than those in normal ovarian tissues (P<0.05). 4) There are no obvious relationships to ages, histological types (P>0.05), but there are obvious relationships to histological grades, differentiation grades, lymph node metastasis and ascites (P<0.05).5) Although the positive expression rates of TβR-Ⅱand Smad4 are both lower in epithelial ovarian tumors, there is no obvious relationship between TβR-Ⅱand Smad4 (P>0.05).3. Conclusion:1) As a dual role factor, TGF-βis overexpressed in epithelial ovarian tumor tissues, which indicate TGF-βmaybe become the forepart assistant diagnostic target of epithelial ovarian tumor.2) As cancer supprepressor genes, TβR-Ⅱand Smad4 are both lowexpressed in epithelial ovarian tumor tissues indicate that the losses of TβR-Ⅱand Smad4 will lead to the interruption of TGF-βpathway and induce epithelial ovarian tumor.3) Although TβR-Ⅱand Smad4 are both lowexpressed in epithelial ovarian tumor tissues, there is no obvious relationship between them, which indicate that the losses of them maybe occur at the same time or separately to induce the interruption of TGF-βpathway.4) The overexpression of TGF-βand the lowexpression of TβR-Ⅱand Smad4 indicate that TGF-β,TβR-Ⅱand Smad4 maybe become new marker in the field of judging epithelial ovarian tumor.TGF-βpathway relates to the emergence and development of epithelial ovarian tumor, but the exact mechanism is still unknown. With the development of the molecular biology, TGF-β, Smads and other downstream molecules may become new action points for diagnosis and therapy for every tumor. It will be a new method to therapy every tumor with genes by intervening the main molecules of TGF-βpathway.