The Relationship Between Intestinal Microflora And 2 Diabetes Mellitus In Xinjiang Uygur

Objective:To study difference of intestinal microflora between type 2 diabetes mellitus and euglycemia samples in xinjiang uygur.Methods:30 patients with type 2 diabetes Mellitus(T2DM) were selected and there were 33 subjects with euglycemia as the controls in xinjiang uygur.we collected their samples of feceals and extracted composite DNA of bacterium in these samples.Primers were used to amplify V6 to V8 variable regions of the bacterial 16S rDNA.Eventually,cloning and sequencing the purified PCR products,we analysed the composition of the microbial population.Results:(1) Some bands were appeared between 200bp and 1500bp when performing in agarose gel electrophoresis.There were significant differences on the bands in PCR products of intestinal microflora between T2DM groups and control groups(P＜0.05).(2) There were different gene orders in the intestinal microflora of the two groups with blast soft ware analysised on Genebank.(3) The obtained sequences were compared with sequence of known bacteria listed in the genebank nucleotide sequence database by using blast soft ware,euglycemia sample's similarity were 100%compared with Uncultured Bacillus sp.(AY242538)and Lactococcus lactis(AM849109),T2DM sample's similarity were 90% and 93%.Therefore,The bacteria identified included members of Uncultured Bacillus sp. and Lactococcus lactis in euglycemia sample.Conclusions:Compared with the samples, there were different intestinal microfloras with T2DM and euglycemia samples.T2DM sample lacked two bacterias in intestinal microfloras.