The Meaning Of (1→3)-β-D-glucan-detection In The Early Diagnosis Of Deep Fungal Infection

Because of the aging of population, generally application of antibiotic, GC and immunosuppressant, the development of new treamtents for hematologic malignacies and organ transplants, the incidence rate of deep fungal infection increases in recent years.The clinical situation of deep fungal infection shows non-specificity and multiplicity.The positive rate of fungus culture can not satisfied us, separating and appraising the fungus is still difficult to serve for clinical diagnosis.In addition, the number of high performance and safe antifungal agent is so little that the death rate of deep fungus infection stay at a high level. Now, the fungal infections' diagnosis is very difficult. Definite diagnostic criteria and normal serological diagnosis method is lack.Therefore, it is desirable to have a rapid and accurate method of identification.At present, Much work has been done to help the diagnosis of systemic fungal infection according to the fungus components and the metablites. These ingredient include enolase, (1→3)-β-D- glucan,D-arabitol,mannan, galactosyl- mannan and Cand-Tec etc. Among of the above, detecting of (1→3)-β-D-glucan has already been paid close attention to and become the the most dramatic method to diagnose invasive fungal infections.The essential component of fungul cell wall is polycose. (1→3)-β-D-glucan is 50% percent of the total.It is different from fungul, there is no such ingredient in the cell wall of bacterium, virus, human body cell and other pathogenic bacterias.So, in abstracto, when true fungus infect human blood and deep tissue, through phagocytic phagocytosis and digestion, (1→3)-β-D-glucan release form the cell wall so that we can detect the more amount of (1→3)-β-D-glucan in hunman blood and other hunman body fluids such as urine, CSF, peritoneal fluid and pleural fluid.This study detected the levels of (1→3)-β-D-glucan in hunman blood, urine, pucture fluid, peritoneal and pleural fluid and alveoli irrigating solution by SLP Reagent Set to study the meaning of (1→3)-β-D-glucan-detection in the early diagnosis of systemic fungal infection. It was reported first in China to do so.The results suggest that we can quickly judge the level of (1→3)-β-D-glucan by visual test of SLP Reagent Set to help us diagnose deep fungal infection. The sensitivity and the pecificity were 85.1%, 100%. In addition, we carried out quantitative determination by a micro- plate reader. The concentration of (1→3)-β-D- glucan in the sample was obtained according to a standard curve. The results shows extremely high content of (1→3)-β-D-glucan in patients'blood, urine, pucture fluid, peritoneal and pleural fluid and alveoli irrigating solution. Just the opposite to it, the concentration of (1→3)-β-D- glucan in healthy blood were lower.According to the research results, (1→3)-β-D-glucan was a signifi- cance level on specific diagnosis of Deep Fungal Infection. The method of SLP test is convenient, quick, exact and sensitive, has a high value in the early diagnosis of systemic fungal infection and can be used in the surveillance of the effective treatment.