Study Of The Neuroprotective Effect Of Aspirin And Hirudin After Rat Ischemic/reperfusion Injury

Object:Through the model of focal cerebral ischemia/reperfusion in rats pro-dealed with asipirin and r-hirudin,to study the effect of the two drags after focal cerebral iscbemia on neurological outcome,infarct size,and expression of proapoptotic and antiapoptotic proteins Bax,Bcl-2 and Bax/Bcl-2,respectively.Methods:The 80 clean male rats are randomly divided into five groups:1) sham operation group(n=16):normal sodium;2) control group(n=16):normal sodium;3) aspirin treated group(n=16):aspirin 80mg/kg;4) r-hirudin treated group(n=16):r-hirudin 0.5mg/kg;5) aspirin and r-hirudin treated group(n=16):r-hirudin 0.5 mg/kg + aspirin 80mg/ kg,intraperitoneal injected respectively with the medicine by the divided groups with the same volume liquid at the same time,qd.After pro-treated for three days.these rats were operated middle cerebral artery occlusion(MCAO),and then the rats were evaluated with Bederson measuring scale.The time of reperfusion is 2 hours after ischemia.After reperfusion for 24 hours,the animals were then killed,and brains underwent either 2,3,5-triphenyltetrazolium chloride (TTC) staining for assessment of infarct volume or paraffin embedding for morphology and immunohistochemistry(Bax,Bcl-2),HE dyeing.Results:Ischemic damage is mainly restrict at ipsilateral frontal lobe,parietal cortex and dorsal caudate putamen provided blood by middle cerebral artery(MCA).Neurological deficit was improved in aspirin plus r-hirudin treated group versus control group,aspirin treated group or r-hirudin treated group respectively after refusion 6h or 24h(P＜0.05),and infarct volume ratio was decreased in aspirin plus r-hirudin-treated animals versus other three groups(P＜0.05).In the cores of ischemic damage,neuron dyeing is very light,the amount of neuron decrease, the drug treated group was less serious than control group.Bcl-2,Bax positive cell is brown,cytoplasm were dyed.Bax were significantly reduced in aspirin plus r-hirudin treated group versus other three groups(P＜0.05),and Bax/Bcl-2 ratio increase in aspirin plus r-hirudin treated group versus control group or r-hirudin treated group(P＜0.05). Conclusions:1) It can improve neurological deficit,decrease infarct volume ratio and lighten the pathological change through providing aspirin plus r-hirudin post ischemia/reperfusion injury in rats.This study demonstrates a neuroprotective effect of aspirin plus hirudin for ischemical/reperfusion injury when pro-delivered intraperitonealy. 2) The mechanism of neuroprotective effect of aspirin plus hirudin may be partly due to depressing the expression of Bax and increase Bax/Bcl-2 ratio after cerebral ischemia reperfusion in rats.