Antioxidation Of Probucol Pretreatment Against Acute Renal Ischaemia-Reperfusion Injury In Rats

Objective:To investigate the total activity of superoxide dismuase(SOD) and level of malondialdehyde(MDA)in serum,urine and renal tissues and the effect of probucol pretreatment on male Wistar rat with ischemia-reperfusion injury.Methods:90 wistar rats were randomly divided into 3 groups:false operating group,ischemia-reperfusion group and probucol pretreated group,each involved 30 rats.The animals in the false operating group and ischemia-reperfusion group were fed daily with standard rat diet,rats in the pretreated group were fed daily with standard rat diet+probucol (500mg/kg.d,twice a day per animal).After three weeks,rat renal ischemiareperfusion model was performed by placing the atraumatic microvascular clamp in both renal artery for 60 minutes.The total activity of superoxide dismuase(SOD)and level of malondialdehyde(MDA)in serum,urine and renal tissues were detected at various intervals with chromatometry.We also examined possible association between the total activity of SOD and level of MDA and other correlate index.Result:(1)The renal/weight index of ischemia-reperfusion group increased from 12 hour to 72 hour post-reperfusion(p＜0.05),and reached its peak in 48 hour.The renal/weight index of probucol group increased from 24 hour to 72 hour post-reperfusion(p＜0.05),and reached its peak in 48 hour.(2)In ischemia-reperfusion group,plasma levels of BUN,Cr and kalium increased from 6 hour to 72 hour post-reperfusion(p＜0.05),reached its peak in 48 hour.In probucol pretreated group,the plasma levels of BUN increased form 6 hour to 48 hour post-reperfusion(p＜0.05),and reached its peak in 24 hour;the plasma levels of Scr increased form 6 hour to 72 hour post-reperfusion(p＜0.05),and reached its peak in 24 hour;the plasma levels of kalium increased from 6 hour to 72 hour post-reperfusion(p＜0.05),and reached its peak in 48 hour.The levels of BUN,Cr and kalium were lower in probucol pretreated group as compare to ischemia-reperfusion group.(3)Plasma levels of the total activity of SOD decreased from 6 hour post-reperfusion(p＜0.05),there was no significant difference between probucol pretreated group and ischemia-reperfusion group(P＞0.05).In ischemia-reperfusion group,the total activity of SOD in renal tissues increased form 6 hour to 72 hour post-reperfusion(p＜0.05),and reached its peak in 48 hour.In probucol pretreated group,the total activity of SOD in renal tissues increased form 6 hour to 72 hour post-reperfusion(p＜0.05),and reached its peak in 12 hour.The changs of the total activity of SOD in serum and renal tissues were lower in probucol pretreated group as compare to ischemia-reperfusion group(p＜0.01).(4)The levels of total activity of SOD in urine increased from 6 hour to 24 hour post-reperfusion(p＜0.05),and reached its peak in 12 hour.In 48 hour post-reperfusion,the level of total activity of SOD in urine was higher in probucol pretreated group than in the ischemiareperfusion group(P＜0.05).(5)In ischemia-reperfusion group,the levels of MDA in serum and renal tissues increased from 6 hour to 72 hour postreperfusion (p＜0.05),and reached its peak in 24 hour.In probucol pretreated group,the levels of MDA in serum increased from 6 hour to 72 hour post-reperfusion(p＜0.05),and reached its peak in 12 hour;the levels of MDA in renal tissues increased from 6 hour post-reperfusion(p＜0.05),and there was no significant difference between the hours post-reperfusion(P＞0.05).The levels of MDA in urine increased from 6 hour post-reperfusion(p＜0.05).And in 48 hour post-reperfusion,the level of MDA in urine was lower in probucol pretreated group as compare to the ischemia-reperfusion group(P＜0.05).Conclusion:(1)Renal I/R model of rat can be achieved by clamping both renal artery of rat for 60 minutes.(2)In renal IRI model,oxidative stress contribute to the development of ischemia-reperfusion injury in rats.(3)In renal IRI model,probucol can lessen the damage of kidney and promote functional restoration of the renal tissue.(4)In renal IRI model,pretreatment of probucol can protect renal function after ischemia-reperfusion injury,it can decrease the level of MDA and increase the activity of SOD.